We narrowed to 11,637 results for: NSI

1. pAC-EsaR-I70V/D91G

   Plasmid

   #47644

   Purpose

   pAC-EsaR with a208g and a272g mutations in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-I70V/D91G
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Isoleucine 70 to Valine and Aspartic acid…

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pGL3_zfh1_CP-candidate_luc+

   Plasmid

   #86391

   Purpose

   Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer
   

   Use

   Luciferase

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pAC-EsaR-D91G/V220A

   Plasmid

   #47648

   Purpose

   pAC-EsaR with a272g and c659t mutations in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-D91G/V220A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 91 to Glycine, and Valine 2…

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pVG122

   Plasmid

   #211765

   Purpose

   Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   strongLOV
   

   Use

   For single-copy integration (if cut with pmei)

   Tags

   SV40 NLS and VP16

   Expression

   Yeast

   Mutation

   compared to EL222, strongLOV contains Glu84Asp mu…

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSTAP-seq_fly-zfh1

   Plasmid

   #86381

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSTAP-seq_fly-tj

   Plasmid

   #86386

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster tj enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSTAP-seq_fly-ssp3

   Plasmid

   #86382

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ssp3 enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSTAP-seq_fly-ham

   Plasmid

   #86383

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster ham enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-sgl

   Plasmid

   #86384

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster sgl enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSTAP-seq_fly-ncm

    Plasmid

    #86385

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ncm enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-EsaR-I70V/D91G/V220A

    Plasmid

    #47647

    Purpose

    pAC-EsaR with a208g, a272g, c659t mutations in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-I70V/D91G/V220A
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Isoleucine 70 to Valine, Aspartic acid 91…

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSP1-mp53AS

    Plasmid

    #20896

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

    Insert

    p53AS (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    5' natural EcoRV and SacI restriction sites …

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pEXPA10-NLS-3xmCitrine

    Plasmid

    #192523

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    EXPANSIN A10 (EXPA10 Mustard Weed)
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    EXPA10

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Expression

    Mammalian

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eMscL WT

    Plasmid

    #107454

    Purpose

    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

    Depositor

    Francesco Difato

    Article

    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

    Insert

    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    

    Use

    Adeno-associated viral

    Tags

    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

    Expression

    Mammalian

    Promoter

    human synapsin 1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid

    #229509

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    RbcX2
    BSD2
    RbcX1
    

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. eMscL G22S

    Plasmid

    #107455

    Purpose

    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

    Depositor

    Francesco Difato

    Article

    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

    Insert

    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    

    Use

    Adeno-associated viral

    Tags

    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

    Expression

    Mammalian

    Mutation

    Bearing a glycine to serine substitution at posit…

    Promoter

    human synapsin 1

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304_miRFP670nano-P2A-ERM-gCam6f

    Plasmid

    #214927

    Purpose

    Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

    Depositor

    Xinnan Wang

    Article

    Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

    Insert

    miRFP670nano-P2A-ERM-gCam6f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMVp

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUW-tetO-wtYAP

    Plasmid

    #84009

    Purpose

    expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    YAP1 (siRNA insensitive) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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