We narrowed to 733 results for: Tcta

1. pJYS2cm_crtYf

   Plasmid

   #85543

   Purpose

   Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

   Plasmid

   #84248

   Purpose

   Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MK1274

   Plasmid

   #71431

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

   Promoter

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJZC603

   Plasmid

   #62317

   Purpose

   sgRNA with 2x PP7 for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x PP7 RNA binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO-Tet-On-HDAC1-shRNA2

   Plasmid

   #90241

   Purpose

   Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJZC545

   Plasmid

   #62313

   Purpose

   sgRNA with 1x MS2 for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 1x MS2 binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-U6-AksgRNA

   Plasmid

   #121955

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b single chimeric gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid

    #73224

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL22-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL22

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJZC583

    Plasmid

    #62314

    Purpose

    sgRNA with 2x MS2 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmU6 ST

    Plasmid

    #188713

    Purpose

    sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-U6-crRNA1

    Plasmid

    #123639

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6_(GLuc)_sgRNA

    Plasmid

    #68422

    Purpose

    Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Gluc sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL3.7 Lmo4.3 shRNA

    Plasmid

    #59292

    Purpose

    shRNA to mouse Lmo4

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

    Insert

    Lmo4.3 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only