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pDEST_FLAG-HUWE1_UBM-mut

Plasmid

#187150

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UIM-mut

Plasmid

#187148

Purpose

mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

A1376G/S1382A

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA-mut

Plasmid

#187146

Purpose

mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

M1328A/F1330A

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_YH/GG

Plasmid

#187123

Purpose

mammalian cell expression of FLAG-HUWE1 Y355G/H356G

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

Y355G/H356G

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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UTS2R-Tango

Plasmid

#66519

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

UTS2R (UTS2R Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298

Plasmid

#187127

Purpose

mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Y355G/H356G, delta3291-3298

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta169-189

Plasmid

#187136

Purpose

mammalian cell expression of FLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta169-189

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

Plasmid

#187135

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

delta169-189

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

Plasmid

#187133

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

delta3896-3951

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

Plasmid

#187129

Purpose

mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I3969A/F3982A

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM/UBM-mut

Plasmid

#187153

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SaMscL (CDelta26)

Plasmid

#79027

Purpose

Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

Depositor

Douglas Rees

Article

Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

Insert

MscL

Use

Tags

6x Histidine Tag

Expression

Mutation

Stop codon placed before 26 amino acids at C Term…

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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p3A2

Plasmid

#234793

Purpose

Mammalian expression of bovine arrestin-2

Depositor

Vsevolod Gurevich

Article

Pan et al J Biol Chem. 2003 Mar 28;278(13):11623-3

Insert

Arrestin-2 (ARRB2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A2-RE

Plasmid

#234794

Purpose

Mammalian expression of bovine arrestin-2 (R169E)

Depositor

Vsevolod Gurevich

Article

Pan et al J Biol Chem. 2003 Mar 28;278(13):11623-3

Insert

Arrestin-2 (ARRB2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

R169E

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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