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pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

Plasmid

#40806

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

MSV-LTR

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
SIN40C.SFFV.mTagBFP2.let-7c

Plasmid

#169310

Purpose

Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

TagBFP2

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.let-7c

Plasmid

#169301

Purpose

Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR-99a

Plasmid

#169304

Purpose

Constitutive overexpression of miR-99a with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-99a (MIR99A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

Plasmid

#169316

Purpose

Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-ctrl

Plasmid

#169312

Purpose

Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

TagBFP2

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1K908E-VSV

Plasmid

#139357

Purpose

Expression of kinase-dead JAK1-K908E protein

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

K908E

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1P1084A-VSV

Plasmid

#139358

Purpose

Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

P1084A

Promoter

CMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G

Plasmid

#169315

Purpose

Doxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

Plasmid

#218460

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues)-wild type (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-125b-2

Plasmid

#169311

Purpose

Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Use

Tags

Cleavable 6 x HIS tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFOS WT-GL3

Plasmid

#11983

Purpose

Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

c-fos promoter (Fos Mouse)

Use

Tags

Luc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pSUMO-β′-COPI-WD40

Plasmid

#180753

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

β′-COPI-WD40 (SEC27 Budding Yeast)

Use

Tags

N-terminal strep tag, His-tag, and SUMO tag

Expression

Bacterial

Mutation

Encodes residues 1-301; deleted 302-889; mutated …

Promoter

T7

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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LP725

Plasmid

#90182

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa residues 561-1826

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LP399

Plasmid

#90176

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-1289

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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LP696

Plasmid

#90177

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1289-1826

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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LP344

Plasmid

#90174

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-370

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-370

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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