We narrowed to 145,972 results for: ins;
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Plasmid#78670PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4
Plasmid#69456PurposeRNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 + SFGFP
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS3
Plasmid#69455PurposeRNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S3
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S3 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tg-UBC (TgTwinScan_2218)
Plasmid#25565PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTg-UBC (TgTwinScan_2218)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)
Plasmid#195048Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
UseTagsExpressionYeastMutationPromoterpADH2, pGPD, and pXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)
Plasmid#195047Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
UseTagsExpressionYeastMutationPromoterpADH2, pGPD, and pXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)
Plasmid#195046Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
UseTagsExpressionYeastMutationPromoterpADH2, pGPD, and pXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-INSR-V5/HIS
Plasmid#201980Purposeexpression of human INSR receptor tyrosine kinase in mammalian cellsDepositorInsertINSR receptor tyrosine kinase (INSR Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 A775_G776insYVMA
Plasmid#40982DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationA775_G776insYVMAPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-H773_V774insNPH
Plasmid#116262PurposeLentiviral expression of EGFR H773_V774insNPHDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationH773_V774insNPHPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXT128_TwinStrep-SUMO-ShCas12k
Plasmid#135524PurposeProtein expression construct for ShCast12kDepositorInsertShCas12k
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-A763_Y764insFQEA
Plasmid#116233PurposeLentiviral expression of EGFR A763_Y764insFQEADepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationA763_Y764insFQEAPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-M766_A767insAI
Plasmid#116279PurposeLentiviral expression of EGFR M766_A767insAIDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationM766_A767insAIPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXT131_TwinStrep-SUMO-ShTniQ
Plasmid#135527PurposeProtein expression construct for ShTniQDepositorInsertShTniQ
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXT129_TwinStrep-SUMO-ShTnsB
Plasmid#135525PurposeProtein expression construct for ShTnsBDepositorInsertShTnsB
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis
Plasmid#232344PurposeExpresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tagDepositorInsertHDGF Like 2 (HDGFL2 Human)
UseTagsGGGS linker, 6xHis and Twin-strep, TEV protease s…ExpressionMammalianMutationTDP-43 regulated cryptic exonPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only