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pICH-roGFP2-synORP1

Plasmid

#191680

Purpose

Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

rredox(roGFP2-synORP1

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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RP156 pUAST EGFP:Gr21a

Plasmid

#59524

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Gr21 (Gr21a Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
R777-E243 Hs.RASGRP1

Plasmid

#70527

Purpose

Gateway ORF clone of human RASGRP1 [NM_005739.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RASGRP1 (RASGRP1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-ORP1

Plasmid

#191677

Purpose

Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-ORP1

Use

Binary agrobacterium ti plasmid

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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MRP1-CD4d3+4-bio

Plasmid

#73120

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

MRP1 (ABCC1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-KanMx

Plasmid

#122923

Purpose

TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-NatMx

Plasmid

#122924

Purpose

TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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RRP1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176957

Purpose

Mammalian expression vector encoding RRP1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RRP1 (RRP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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RP125 pUAST EGFP:Orco

Plasmid

#53441

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Orco

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Orco (Orco Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-V5-DEST-hSFRP1-VF

Plasmid

#189542

Purpose

Protein expression of SFRP1 in mammalian cells.

Depositor

Haian Fu

Insert

secreted frizzled related protein 1 (SFRP1 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5393-CRISPRai-TRP1

Plasmid

#182832

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S600D

Plasmid

#46345

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S600D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S600A

Plasmid

#46344

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S600A

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Arp11 (1-417)

Plasmid

#51398

Purpose

expression of full-length mouse Arp11 in mammalian cells

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
pR I-Ab beta Derp1-117

Plasmid

#113639

Purpose

Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab beta Derp1-117 (H2-Ab1 Mouse)

Use

Tags

6x His tag, fused to Der p 1 (117-127) peptide vi…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge