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Addgene

We narrowed to 10,596 results for: cat.1

Showing: 3161 - 3200 of 10596 results
  1. Fireworks RFP[PTC+] (AVA2626)

    Plasmid
    #85444
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. Fireworks GFP[PTC+] (AVA2600)

    Plasmid
    #85446
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pSUMO1-del

    Plasmid
    #228950
    Purpose
    express the full-length human SAE1:SAE2 heterodimer ( where SAE1 is untagged and SAE2 contains an N-terminal tag : MGSSHHHHHHSQDADLNSRVD, derivated from the Addgene plasmid #52258 pSUMO1)
    Depositor
    Inserts
    UBA 2 (UBA2 Human)
    Aos1 (SAE1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    Promoter
    RBS and T7-lac
    Available since
    May 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Insert
    Mus musculus sphingosine kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. HsSPHK1

    Plasmid
    #118091
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Insert
    Homo sapiens Sphingosine Kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. RS18960_pMQ30_KO_Kan

    Plasmid
    #185393
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS18960 upstream flanking homology region
    RR42_RS18960 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. RS17060_pMQ30_KO_Kan

    Plasmid
    #185396
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS17060 upstream flanking homology region
    RR42_RS17060 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. RS17055_pMQ30_KO_Kan

    Plasmid
    #185398
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. RS02060_pMQ30_KO_Kan

    Plasmid
    #185391
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS02060 upstream flanking homology region
    RR42_RS02060 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. RS28935_pMQ30_KO_Kan

    Plasmid
    #185392
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS28935 upstream flanking homology region
    RR42_RS28935 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. RS18965_pMQ30_KO_Kan

    Plasmid
    #185394
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS18965 upstream flanking homology region
    RR42_RS18965 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. RS18970_pMQ30_KO_Kan

    Plasmid
    #185395
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS18970 upstream flanking homology region
    RR42_RS18970 downstream flanking homology region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. RS35075_pMQ30_KO_Kan

    Plasmid
    #185390
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pZA1

    Plasmid
    #158479
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pZA3

    Plasmid
    #158481
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pZA2

    Plasmid
    #158480
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D489V, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. pZA11

    Plasmid
    #158489
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E no STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. pZA4

    Plasmid
    #158482
    Purpose
    Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D489V, STOP codon
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. pZA10

    Plasmid
    #158488
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon
    Depositor
    Insert
    ZAR1 (ZAR1 Mustard Weed)
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D489V, no STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  21. TFORF2242

    Plasmid
    #141986
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    GATA1 (GATA1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3161 - 3200 of 10596 results