We narrowed to 5,256 results for: pcdna3.1

1. pcDNA-NBid-mMaple-CBid

   Plasmid

   #164049

   Purpose

   Optogenetic manipulation of cell apoptosis negative control

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-mMaple-CBid
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Cenp-B-targeted Aurora B FRET sensor

   Plasmid

   #45232

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOVC1_EcSSPB-nano_X

   Plasmid

   #131212

   Purpose

   Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Flag-YOD1 C160S

   Plasmid

   #49785

   Purpose

   Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

   Insert

   YOD1 (YOD1 Human)
   

   Use

   Tags

   Flag-tag

   Expression

   Mutation

   C160S

   Promoter

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pFOX6-Tier1-PhCMV-TdTomato

   Plasmid

   #169515

   Purpose

   Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven dimeric red fluorescent protein TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNIR-Fb2E7

   Plasmid

   #184681

   Purpose

   NIR-Fb to HIV gp41

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb2E7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

   Plasmid

   #45229

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   non phosphorylatable Mis12-targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T-A mutation in substrate sequence

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRAPa-3xFLAG-pCDNA

   Plasmid

   #201963

   Purpose

   Mammalian expression of Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRPML1-MYC

   Plasmid

   #18824

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML1 (MCOLN1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OSCA1.2-PP-GFP

    Plasmid

    #135390

    Purpose

    Expresses OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    EGFP tag connected to the gene via a precision pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VAChT-SNAP

    Plasmid

    #105318

    Purpose

    VAChT tagged with SNAP

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HIPK1-myc-his

    Plasmid

    #1850

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    HIPK1 (Hipk1 Mouse)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pAL189

    Plasmid

    #22278

    Purpose

    Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TNXXL-CTS68

    Plasmid

    #48343

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS68
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

15. TRPML3-CFP

    Plasmid

    #18834

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML3 (Mcoln3 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q165R, F210Y

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-Pol-II CC

    Plasmid

    #35180

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains C-terminal domain repeats (1-22)X2

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Ilmol

    Plasmid

    #120310

    Purpose

    A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

    Depositor

    Bradley Baker

    Article

    Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

    Insert

    Ilmol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Mammalian_SaAID3S

    Plasmid

    #188651

    Purpose

    Expresses SaAID3S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    nSaCas9-AID3S--UGI
    Sa-sgRNA
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Mammalian_SaAID2S

    Plasmid

    #188652

    Purpose

    Expresses SaAID2S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S-nSaCas9--UGI
    Sa-sgRNA
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-CTE-Luc

    Plasmid

    #60881

    Purpose

    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    Luc-CTE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-Pol-II DC

    Plasmid

    #35181

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C-terminal domain repeats 1-22 and 31-52

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pNIR-Fb4EIG

    Plasmid

    #184683

    Purpose

    NIR-Fb to E.coli dihydrofolate reductase

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb4EIG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Vh-HA-DHFR

    Plasmid

    #20217

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

25. Venus-Gprotein Gamma 9

    Plasmid

    #42201

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    G protein Gamma 9
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pMM545-Tier1-PhCMV-Citrine

    Plasmid

    #169505

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven yellow fluorescent protein citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. [GFP][IRES][Bla]

    Plasmid

    #97194

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. OptoOFF-MxCBD-mFGFR1_623

    Plasmid

    #105825

    Purpose

    Expresses OptoOFF-MxCBD-mFGFR1 (light-inactivated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

    Insert

    OptoOFF-MxCBD-mFGFR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOVC1_L4(EAAAK)3

    Plasmid

    #131211

    Purpose

    Cloning vector containing A-B-C casette with linker L4 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    (EAAAK)3 linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAB303-Tier1-PhCMV-TetR_VPR

    Plasmid

    #169575

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB301-Tier1-PhCMV-rTetR_VPR

    Plasmid

    #169579

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    reverse tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNIR-Fb59H10

    Plasmid

    #184682

    Purpose

    NIR-Fb to HIV p24

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb59H10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOVC1_AtPHYB-S_X

    Plasmid

    #131183

    Purpose

    Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtPHYB-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOVC1_NcVVD-LOV_X

    Plasmid

    #131176

    Purpose

    Expresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    NcVVD-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-GATA2-DNzf

    Plasmid

    #1291

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S263G and M434V

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pVH227-Tier1-OTetO7-PCMVmin2-SEAP

    Plasmid

    #169563

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP production
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFOX13-Tier1-PhCMV-tGFP

    Plasmid

    #169510

    Purpose

    Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven turbo green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. myc-NLRC5 deltaDD

    Plasmid

    #37511

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

    Insert

    NLRC5 (NLRC5 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    lacking the N-terminal domain (deletion: aa 1-134)

    Promoter

    CMV

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA-GATA2-DC+Nzf

    Plasmid

    #1293

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pDD2703

    Plasmid

    #175079

    Purpose

    Mammalian expression of Flag-tagged KSHV ORF45 E223A-S226A mutant

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E223A, S226A

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge