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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 34,848 results for: CaS;

Showing: 801 - 810 of 34848 results

  1. pK7WGF2::hCas9

    Plasmid
    #46965
    Purpose
    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
    Depositor
    Sophien Kamoun
    Article
    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.
    Insert
    hCas9
    Use
    CRISPR; Plant expression
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    35S
    Available Since
    Aug. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    46965-plasmid-map-sequence-id-66887

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  5. CASR-Tango

    Plasmid
    #66235
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CASR (CASR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66235-plasmid-map-sequence-id-378710

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  7. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  9. pKS diaCas9_sgRNA

    Plasmid
    #74923
    Purpose
    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
    Depositor
    Per Winge
    Article
    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Cas9 module and sgRNA module
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74923-plasmid-map-sequence-id-149703

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  10. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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Showing: 801 - 810 of 34848 results