We narrowed to 7,778 results for: CCH
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Plasmid#180753PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).DepositorInsertβ′-COPI-WD40 (SEC27 Budding Yeast)
TagsN-terminal strep tag, His-tag, and SUMO tagExpressionBacterialMutationEncodes residues 1-301; deleted 302-889; mutated …PromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
Plasmid#89607PurposeExpression of LIN28A in human cellsDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
TagsAID(71-114)ExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-DVL2deltaDIX
Plasmid#114276PurposeTET-inducible expression of DVL2 protein with a deletion of its DIX-domainDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
Plasmid#126739Purposeyeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2, acs1*, ald6
Tags6xHis,MycExpressionYeastMutationT3542C and A30S, S629N (please see depositor comm…PromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-pTEF-Sc_IntAct-tCYC
Plasmid#248980PurposeLow-copy centromeric plasmids which expresses Saccharomyces cerevisiae IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)DepositorInsertSc_IntAct
TagsALFA tagExpressionYeastPromoterTEFAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-pAct1-Sp_IntAct-tADH1
Plasmid#250862PurposeLow-copy centromeric plasmids which expresses Schizosaccharomyces pombe IntAct (actin internally tagged with ALFA tag between T229 and A230 amino acids)DepositorInsertSp_IntAct
TagsALFA tagExpressionYeastPromoterTEFAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Tyr33Ala
Plasmid#208170PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
TagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Arg57Ala
Plasmid#180759PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)DepositorInsertα-COPI-WD40-Arg57Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Arg57Ala, Leu1…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Tyr139Ala
Plasmid#180761PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)DepositorInsertα-COPI-WD40-Tyr139Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Tyr139Ala, Leu…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-dnTCF4
Plasmid#114277PurposeTET-inducible expression of a dominant negative form of the TCF4 transcription factorDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
TagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
ExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1 F121A
Plasmid#99506PurposeLentiviral expression of mutant Becn1 leading to hyperactive autophagyDepositorAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralExpressionMammalianPromoterhEF1aAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only