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We narrowed to 14,223 results for: Cas9

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Showing: 1601 - 1620 of 14223 results

  1. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

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  4. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

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  5. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Publication
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109219-plasmid-map-sequence-id-260727

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  6. pspCas9-HF-tetra-com-vector

    Plasmid
    #132555
    Purpose
    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    132555-plasmid-map-sequence-id-259534

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  7. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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  14. pAT15542_nCBE-SuperFi-Cas9

    Plasmid
    #184372
    Purpose
    Mammalian expression of SuperFi-Cas9 CBE base editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCBE-SuperFi-Cas9
    Use
    CRISPR
    Tags
    3X FLAG, SV40 NLS, and UGI
    Expression
    Mammalian
    Mutation
    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…
    Promoter
    EF-1α core promoter
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184372-plasmid-map-sequence-id-362132

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  15. pAT15544_nABE-SuperFi-Cas9

    Plasmid
    #184374
    Purpose
    Mammalian expression of SuperFi-Cas9 ABE7 base editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nABE-SuperFi-Cas9
    Use
    CRISPR
    Tags
    FLAG, SV40 NLS, and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…
    Promoter
    EF-1α core promoter
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184374-plasmid-map-sequence-id-362135

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  17. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Publication
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96921-plasmid-map-sequence-id-181715

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  19. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104406-plasmid-map-sequence-id-204465

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  20. p426_Cas9_gRNA-HIS3b

    Plasmid
    #87402
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87402-plasmid-map-sequence-id-508301

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Showing: 1601 - 1620 of 14223 results