We narrowed to 3,098 results for: actin

1. pcDNA3-LifeAct-FRB-Venus-173-N

   Plasmid

   #87616

   Purpose

   FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-Venus173-N
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LifeAct-TagBFP-DiB-RM

   Plasmid

   #168782

   Purpose

   Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

   Depositor

   Jens Meiler

   Article

   Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

   Insert

   LifeAct-TagBFP-DiB-RM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLNCX2-lifeact-mAPPLE

   Plasmid

   #89820

   Purpose

   mAPPLE labeled fluorescent actin

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   lifeact-mAPPLE
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAct:dCas9-scFv

   Plasmid

   #78900

   Purpose

   Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   scFV-VP64
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA-T-intron-G

   Plasmid

   #36887

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (i.e., ATG, start codon as a first exon)
   GFP
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides before nucleotide 275 and ins…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA-G-intron-T

   Plasmid

   #36885

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGs-Hoxc8

   Plasmid

   #45555

   Depositor

   Jeremy Dasen

   Article

   Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

   Insert

   Hoxc8 (HOXC8 Chicken)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV / b-actin

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCA-ATG-intron-tdT3Myc

   Plasmid

   #36883

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tdTomato-3Myc
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   beta-globin intron containing a loxP site inserte…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos2-FilaminA-C-14

   Plasmid

   #57378

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   FilaminA (FLNA Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pONSY-Lifeact:mCherry

    Plasmid

    #111876

    Purpose

    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Lifeact fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAct:MS2-p65-HSF

    Plasmid

    #78902

    Purpose

    Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-p65-HSF
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PA-mCherry-miniSOG-Lifeact-7

    Plasmid

    #54846

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry-miniSOG-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

    Plasmid

    #132684

    Purpose

    AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 2 (Pm20d2 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT234_(pCA-G-intron-tTA2)

    Plasmid

    #36874

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235298

    Purpose

    ComMAND closed-loop circuit regulating mRuby2, CAG-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (synthetic cytomegalovirus early enhancer + c…

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-mRuby2-bGH

    Plasmid

    #235296

    Purpose

    ComMAND base gene regulating mRuby2, CAG-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (synthetic cytomegalovirus early enhancer + c…

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GT_CAG-ITPKA-mScarlet-I

    Plasmid

    #229778

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    ITPKA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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