Addgene

We narrowed to 11,654 results for: cel.2

Showing: 1601 - 1620 of 11654 results

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pPPI4-Strep-SNAP-CD86-His10

Plasmid

#223570

Purpose

Protein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

CD86 (CD86 Human)

Use

Tags

His6 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Id2

Plasmid

#70764

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

inhibitor of DNA binding 2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pv6_TAF3_2xPhd

Plasmid

#179399

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3_2xPhd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2

Plasmid

#197117

Purpose

Expression of Myc-tagged PSK2 / TAOK1 in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

W157E

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Use

Tags

GST

Expression

Bacterial

Mutation

F158E

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Cox8A Control Plasmid

Plasmid

#101129

Purpose

Expression of SNAP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Use

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCLIPf-Cox8A Control Plasmid

Plasmid

#101130

Purpose

Expression of CLIP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Use

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCD86-mEGFP

Plasmid

#190745

Purpose

Expression of mEGFP-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCJ1022

Plasmid

#228762

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Pkd2 gRNAs (Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCJ1021

Plasmid

#228761

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 gRNAs (Ift88 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1

Plasmid

#181812

Purpose

Expresses human MUC2 D1, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Use

Tags

6xHistadine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-PuroR-LoxP

Plasmid

#227502

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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Mtp-p53-ECFP

Plasmid

#53961

Purpose

Expresses CFP tagged p53 in mammalian cells

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

p53 (TP53 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCLJ-P2F-Frt-PGK-Puro-Frt

Plasmid

#227501

Purpose

Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Fgf5 promoter

Use

AAV and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-NeoR-LoxP

Plasmid

#227503

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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