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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBIS

Plasmid

#35150

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Promoter

LacUV5,

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV4-p105

Plasmid

#23288

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p105 (NFKB1 Human)

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

LMNA-gRNA1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1

Plasmid

#45903

Depositor

Louise Larose

Article

Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

Insert

Nck-1 (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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