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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab4 DN

Plasmid

#37691

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pJBL719

Plasmid

#140389

Purpose

Expresses QacR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-QacR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL721

Plasmid

#140391

Purpose

Expresses TtgR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TtgR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-myr-eGFP-SV40pA-FKF

Plasmid

#119866

Purpose

Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-myr-tdTomato-SV40pA-FKF

Plasmid

#119867

Purpose

Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hPlekhm1-C/pIRES puro Glue (S778-A1056)

Plasmid

#73456

Purpose

Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab3 WT

Plasmid

#37687

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab10 DN

Plasmid

#37709

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T23N (dominant negative)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV-Cx43

Plasmid

#69442

Purpose

mammalian expression of rat Cx43

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

Insert

Cx43 (Gja1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

OR2-OR1-Pr

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFusX4-35

Plasmid

#63444

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NI NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab10 CA

Plasmid

#37710

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q68L (constitutive active)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab7 WT

Plasmid

#37699

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO

Plasmid

#73448

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Article

Yang et al J Cell Physiol. 2008 May;215(2):497-505.

Insert

Ocstamp (Ocstamp Mouse)

Use

Tags

V5-6xHis

Expression

Mammalian

Mutation

N162D

Promoter

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP YFP Rab18 WT

Plasmid

#37717

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pFusX1-31

Plasmid

#63249

Purpose

Encodes RVD positions 1-3 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab8 WT

Plasmid

#37705

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only