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EF.delmFasL.CMV.GFP

Plasmid

#17620

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Insert

delFasL (Fasl Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Noncleavable mouse delFasL . Deleted the exon 2 r…

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6B

Plasmid

#15473

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6B (Pard6b Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pRVL-3

Plasmid

#104581

Purpose

Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)

Expression

Mammalian

Mutation

L234A/L235E/G237A and E318A/K320A/K322A

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rubicon

Plasmid

#21636

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Rubicon (1700021K19Rik Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

*(see below)

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBS mFgfr1 (CT#92)

Plasmid

#14005

Depositor

Cliff Tabin

Insert

Fgfr1 (Fgfr1 Mouse)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pVAX1-cGAS

Plasmid

#196624

Purpose

Express mouse cGAS protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

cGAS (Cgas Mouse)

Expression

Mammalian

Mutation

WT

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)

Plasmid

#114214

Purpose

AAV vector that targets expression to GABAergic inhibitory neurons in rodent cortex with 98% selectivity

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD

Plasmid

#22729

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted amino acids 14-301

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenC124S-Puro

Plasmid

#135677

Purpose

Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Mutation

C124S

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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Plxna1-Fc-His

Plasmid

#72122

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3

Plasmid

#58389

Purpose

Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

Plasmid

#176278

Purpose

Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-Brg1

Plasmid

#25855

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brahma related gene 1 (Smarca4 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

Plasmid

#216386

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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FpG5

Plasmid

#69443

Purpose

lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT3mts

Plasmid

#195577

Purpose

Expresses STAT3 with mitochondrial targeting sequence

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Cox8 presequence

Expression

Mammalian

Promoter

CMV enhancer and promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Mouse, Glycine max)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBS mShh (CT#258)

Plasmid

#13999

Depositor

Cliff Tabin

Insert

Shh (Shh Mouse)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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