We narrowed to 5,624 results for: pcas

1. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199453

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA31-pCS2-CMV-stdPCP-p65-HSF1

   Plasmid

   #199462

   Purpose

   Expression of stdPCP-PH in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   stdPCP-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA30-pCS2-CMV-stdMCP-p65-HSF1

   Plasmid

   #199461

   Purpose

   Expression of stdMCP-PH in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   stdMCP-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

   Plasmid

   #199452

   Purpose

   CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

   Plasmid

   #199460

   Purpose

   Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   Zebrafish NarTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

   Plasmid

   #198765

   Purpose

   expression of hOCT4 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

   Insert

   hOCT4 236D (POU5F1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S236D

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

   Plasmid

   #195502

   Purpose

   Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

   Plasmid

   #188975

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g1 guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

   Plasmid

   #188976

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g2 guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-MBP-Foldon-ATG9 (801-839)

    Plasmid

    #188089

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (801-839)

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-MBP-Foldon-ATG9 (723-839)

    Plasmid

    #188088

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (723-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-MBP-Foldon-ATG9 (828-839)

    Plasmid

    #188090

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (828-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid

    #179527

    Purpose

    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 K233A/K234A/K253A/K263A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed lysines 233, 234, 253, and 263 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166133

    Purpose

    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

    Plasmid

    #154183

    Purpose

    expresses squirrel monkey CHMP3(155) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3(155)
    

    Use

    Tags

    FLAG, Strep-Tag II, and preScission site

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 155

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid

    #174139

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA

    Plasmid

    #108820

    Purpose

    Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa252 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA

    Plasmid

    #108822

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA

    Plasmid

    #108800

    Purpose

    Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa397 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA

    Plasmid

    #108804

    Purpose

    Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa572 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

    Plasmid

    #117325

    Purpose

    CRISPR-Cas9 for miR-124-2/3, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

    Plasmid

    #117324

    Purpose

    CRISPR-Cas9 for miR-124-1, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1-3' (MIR124-1 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

    Plasmid

    #117323

    Purpose

    CRISPR-Cas9 for miR-124-3, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-3-5' (MIR124-3 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

    Plasmid

    #117322

    Purpose

    CRISPR-Cas9 for miR-124-1/2, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107321

    Purpose

    Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1333S) is fused to SaCas9

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid

    #101191

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5(K918A/V922A/R925A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid

    #101187

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid

    #101189

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge