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pGL3-alphaMHC 3300bp
Plasmid#51934Purposeluciferase reporter for rat alpha-MHC promoterDepositorInsertalpha MHC (Myh6 Rat)
UseLuciferaseTagsluciferaseExpressionMutation-2930 to +410 promoter regionPromoteralpha MHCAvailable sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CAG-FLEX-TVA66T-EGFP
Plasmid#64091PurposeExpresses TVA66T-EGFP under the CAG promotorDepositorInsertTVA66T-EGFP
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationGlutamic acid 66 to ThreoninePromoterCAGAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX385 Streptococcus pasteurianus Cas9
Plasmid#68322PurposeStreptococcus pasteurianus Cas9DepositorInsertSpaCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACP-GPI Control Plasmid
Plasmid#101131PurposeExpression of ACP-Tagged GPI anchor in Mammalian cellsDepositorInsertGPI
UseTagsACP-Tag (ACP wt)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP
Plasmid#178433PurposeCAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)DepositorInserttd-sfGFP
UseCre/Lox; Flp/frt, piggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
Plasmid#45779DepositorInsertsigma factor 28
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/TR
Plasmid#110358PurposeMammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systemsDepositorInsertTetracycline repressor
UseRetroviralTagsExpressionMammalianMutationPromotergag truncatedAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cep215-FL
Plasmid#106911PurposeMammalian expression of CEP215DepositorInsertCEP215 (Cdk5rap2 Mouse)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-hASIC1
Plasmid#32790DepositorInsertASIC1 (ASIC1 Human)
UseCloningTagsExpressionMutationPromoterplacAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUS252s
Plasmid#191833PurposeExpresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbonesDepositorInsertfuGFPs
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)
Plasmid#48291DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP2
Plasmid#78120PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP2
UseTagsKDEL and calreticulin signal peptideExpressionMammalianMutationD24N/D119A in aequorin moietyPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorInsertAFB2 (AFB2 Mustard Weed)
UseTagsExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSV40-GUS-P2A-Hygro
Plasmid#106480PurposeExpression of GUS and Hygromycin resistance gene from SV40 promoterDepositorInsertsbeta-glucuronidase
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pf-GMPS
Plasmid#36899PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-GMPS (PF10_0123 Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLENTI_TetOn_Hu_MAD2(WT)
Plasmid#136347PurposeLentiviral inducible expression (TetOn) of human MAD2DepositorInsertMAD2L1 (MAD2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHuR CDS
Plasmid#110426PurposeTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.DepositorInsertELAVL1 HuR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF79 H-2D(b) (CD8 mut)
Plasmid#180453PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b) (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, replacement of transmembrane domain with e…PromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSL1423
Plasmid#194904PurposepSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparumDepositorInsertTurboID
UsePlasmodium falciparumTagsGFPmut2ExpressionMutationPromoterNone. This allows a C-terminal fusion to a protei…Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FFTTH--GFP-Amotl2
Plasmid#112833Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX396 Parvibaculum lavamentivorans Cas9
Plasmid#68333PurposeParvibaculum lavamentivorans Cas9DepositorInsertPlCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA
Plasmid#112562PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-DsbA(AA20-208) and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-LacZ-BbsI
Plasmid#114671PurposeFor one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expressionDepositorInsertLacZ
UseTagsExpressionMammalianMutationPromoterLac promoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG497 mito-iFAST
Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX377 Corynebacter diphtheriae Cas9
Plasmid#68314PurposeCorynebacter diphtheriae Cas9DepositorInsertCdCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAP-GFP-Amotl2
Plasmid#112835Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsGFP and TAPExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu MAD2(WT)
Plasmid#136343PurposeHuman MAD2 cDNA in Gateway pENTRDepositorInsertMAD2L1 (MAD2L1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)
Plasmid#48292DepositorTypeEmpty backboneUseTagsHis6 and ThioredoxinExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX405 Neisseria meningitidis Cas9
Plasmid#68702PurposeNeisseria meningitidis Cas9DepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab27A
Plasmid#166432PurposeRab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab27A
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV expression vector with BioBrick polypromoer restriction sites (14-C)
Plasmid#48309DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab11A
Plasmid#166430PurposeRab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab11A
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS-Red-N1 GABAA beta3
Plasmid#109143PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.DepositorInsertGABAa receptor
UseTagsRFPExpressionMammalianMutationbeta3 subunit of the GABAa receptorPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only
