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We narrowed to 18,759 results for: nls

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Showing: 3281 - 3320 of 18759 results

  2. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1)(RTW1352)

    Plasmid
    #114081
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114081-plasmid-map-sequence-id-231038

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  7. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220612
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220612-plasmid-map-sequence-id-441117

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  9. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  14. pMini-CMV-NLS-R-IscB-VR4-NLS (D60A, H243E, H269N, R270Q)_T2A_mCherry_U6-ωRNA

    Plasmid
    #246431
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. ssRNase enhancing mutations (H243E, H269N, R270Q) included.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    O.gue IscB-VR4 (H243E, H269N, R270Q) with RuvC dead mutations (D60A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    246431-plasmid-map-sequence-id-496263

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  18. miCBE

    Plasmid
    #205413
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205413-plasmid-map-sequence-id-416749

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  21. pEG302 5aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #115482
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115482-plasmid-map-sequence-id-238819

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  22. mCherry-PCNA-19-NLS-4

    Plasmid
    #55116
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NM_002592.2 (PCNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197025
    Purpose
    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized RhCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197025-plasmid-map-sequence-id-388959

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  27. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Publication
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    4xSV40 NLS and 10xHis-CL7-2xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

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  30. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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  32. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65768-plasmid-map-sequence-id-112655

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  35. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

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  39. DECOR

    Plasmid
    #219545
    Purpose
    Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DECOR
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219545-plasmid-map-sequence-id-443101

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  40. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

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Showing: 3281 - 3320 of 18759 results