We narrowed to 6,530 results for: nls

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MBP-hnRNPA2_LC

Plasmid

#98661

Purpose

Expresses MBP-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

Plasmid

#101177

Purpose

Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES-mannosidase II-mTagBFP2

Plasmid

#165460

Purpose

Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

Partial ManII-mTag2BFP fusion (Man2a1 Mouse)

Tags

Str-KDEL and mTag2BFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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VRQR-HF1 variant

Plasmid

#138563

Purpose

Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRQR-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pST1374-dCas9-GCN4

Plasmid

#113026

Purpose

Expresses dCas9-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D10A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_deltaLC

Plasmid

#98664

Purpose

Expresses MBP-tagged hnRNPA2 without the LC domain

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

G190Stop

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1518

Plasmid

#205981

Purpose

FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX 4t1 RARalpha (1-187)

Plasmid

#35558

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Mutation

aa 1-187

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pBabe 3xFLAG human Bmf

Plasmid

#17242

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 RARalpha (186-462)

Plasmid

#35559

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Mutation

aa 186-462

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
MBP-hnRNPA2_FL_D290V

Plasmid

#98663

Purpose

Expresses MBP-tagged full length hnRNPA2 with D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha (T181A,S445A,S461A)

Plasmid

#35556

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Mutation

T181A, S445A, S461A

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
TC1464

Plasmid

#164881

Purpose

CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

dcas13d-ADARdd

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe 3xFLAG mouse Bmf

Plasmid

#17243

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pST1374-GCN4-H840A

Plasmid

#113024

Purpose

Expresses GCN4-H840A in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-H840A (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mouse Bmf

Plasmid

#17244

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
DNMT3ACD-CRY2-EGFP

Plasmid

#82556

Purpose

Encodes DMNT3 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pST1374-H840A-GCN4

Plasmid

#113027

Purpose

Expresses H840A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

H840A-GCN4 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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TET1CD-CRY2-EGFP

Plasmid

#82555

Purpose

Encodes TET1 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

TET1 (TET1 Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe human Bmf

Plasmid

#17240

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
hnRNPA2_LC_P298L_S285C

Plasmid

#104469

Purpose

express His tagged P298L S285C hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His

Expression

Bacterial

Mutation

P298L S285C

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe mouse Bmf

Plasmid

#17241

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pLVXNeo_BGN-SBP-mSc

Plasmid

#234884

Purpose

Expresses mScarlet-i and SBP tagged human biglycan for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Article

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

Biglycan (BGN Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

See Depositor Comments below

Promoter

CMV

Available Since

March 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVXNeo_SPARC-mSc-SBP

Plasmid

#234885

Purpose

Expresses mScarlet-i and SBP tagged human SPARC for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Article

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

SPARC (SPARC Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-FUS-RGG3

Plasmid

#243672

Purpose

Expresses MBP-tagged FUS RGG3 domain in bacteria

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2021 Nov;28(11):923

Insert

FUS-RGG3 (FUS Human)

Tags

Maltose binding protein

Expression

Bacterial

Mutation

WT

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
6xHis-FUS-LCdel

Plasmid

#243671

Purpose

Expresses 6xHis-tagged FUS with LC domain deletion in bacteria

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2021 Nov;28(11):923

Insert

FUS-delLC (FUS Human)

Tags

6xHis

Expression

Mammalian

Mutation

delete LC domain

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW21K_1Ti1

Plasmid

#177291

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW27K_7Ti1

Plasmid

#177294

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTSK56K_3G7

Plasmid

#177301

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTSK65K_8G7

Plasmid

#177303

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUS

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

uidA

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW07K_0G5

Plasmid

#177279

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW08K_0C5

Plasmid

#177280

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Pcat-cat

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 6,530 results for: nls