We narrowed to 5,867 results for: nls

1. pcDNA3-N-Flag-NLRP3 91-710

   Plasmid

   #75140

   Purpose

   Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 91-710

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-N-Flag-NLRP3 1-90

   Plasmid

   #75137

   Purpose

   Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 1-90

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Icam 2 SaCas9 YAP sgRNA1

   Plasmid

   #99734

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Icam 2 SaCas9 YAP sgRNA3

   Plasmid

   #99736

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-PEmax-P2A-EGFP (LM1589)

   Plasmid

   #223136

   Purpose

   pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized PEmax-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

   Expression

   Mammalian

   Mutation

   M-MLV mutations from PE2 (Anzalone et al. Nature …

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHygG2

   Plasmid

   #200066

   Purpose

   hygR selection nls-mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGADCg

   Plasmid

   #20161

   Depositor

   Peter Uetz

   Article

   Stellberger et al Proteome Sci. 2010 . 8():8.

   Type

   Empty backbone

   Use

   Tags

   Gal4 AD and SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVITRO1-SS-288

   Plasmid

   #68788

   Purpose

   N Trans V2

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   N-Terminal Sensor 1 V2
   

   Use

   Tags

   NLS and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVITRO1-SS-257

    Plasmid

    #68774

    Purpose

    C Trans V2

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-terminal Sensor 1 V2
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNXRVb-HACre

    Plasmid

    #78401

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    hygMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNXRVc-HACre

    Plasmid

    #78400

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    natMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Plasmid

    #68337

    Purpose

    Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Depositor

    Feng Zhang

    Insert

    St3Cas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPS1372

    Plasmid

    #1353

    Depositor

    Pamela Silver

    Article

    Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

    Insert

    pADH-NLS-NES-GFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pBW121-SS-273

    Plasmid

    #68763

    Purpose

    ZF9-TF-V2 with N-FLAG and C-HIS

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    ZF9-TF-V2
    

    Use

    Tags

    HIS, NLS, and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNXRVbt-HACre

    Plasmid

    #78402

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    HyTkAX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

    Plasmid

    #236743

    Purpose

    Constitutive expression of Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
    EF1a-hygro-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. GLuc VLP

    Plasmid

    #182213

    Purpose

    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IRES-BLAST PHGDH

    Plasmid

    #154917

    Purpose

    Expresses human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNXRVct-HACre

    Plasmid

    #78403

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    TKnatAX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVITRO1-SS-255

    Plasmid

    #68773

    Purpose

    C Trans V1

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-Terminal Sensor 1 V1
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVITRO1-SS-259

    Plasmid

    #68775

    Purpose

    C Trans V3

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    C-Terminal Sensor 1 V3
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pG14-MS2-GFP

    Plasmid

    #27117

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2-binding protein
    

    Use

    Tags

    GFP, HA, and NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FRP2370

    Plasmid

    #127576

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRP2365

    Plasmid

    #127575

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNXRVa-HACre

    Plasmid

    #78398

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p3s-Cas9HC

    Plasmid

    #43945

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA epitope and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FRP2371

    Plasmid

    #127577

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pVITRO1-SS-247

    Plasmid

    #68761

    Purpose

    ZF9-TF-V2 with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9-TF-V2
    mCherry
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-ACME

    Plasmid

    #186717

    Purpose

    ACME dual-deaminase CRISPR base editor

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    ACME
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FRP2372

    Plasmid

    #127578

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS406-CMV-lacI-3FLAG

    Plasmid

    #83410

    Purpose

    Expression of lacI in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    lacI repressor
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pISV-CRISPR/Cas9PGmUbi

    Plasmid

    #209189

    Purpose

    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

    Depositor

    Christian Staehelin

    Inserts

    Cas9
    DsRed(deltaB)
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    missing BsaI site and plant-codon optimized with …

    Promoter

    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pME-24xMBS

    Plasmid

    #86246

    Purpose

    Middle entry vector for generating MBS tagged RNAs

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    NLS-tdMCP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMVJ-scTALEN-ABL1-R

    Plasmid

    #61695

    Purpose

    Expresses a single-chain TALEN targeting ABL1 site in human cells

    Depositor

    Huimin Zhao

    Article

    Sun et al Mol Biosyst. 2014 Mar 4;10(3):446-53. do

    Insert

    scTALEN-ABL1-R
    

    Use

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pRVV655

    Plasmid

    #119019

    Purpose

    Drives TP901-1 recombinase expression in the D. melanogaster germ line

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 recombinase
    

    Use

    Tags

    SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge