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Plasmid#124535PurposeFoxo Renilla luciferase reporterDepositorInsertFOXO Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pRSETB
Plasmid#48207PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTags6xHIS, ECFP, and cpCit174ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
hRIP1 HA GFP wt
Plasmid#41396DepositorInsertRIP1 (RIPK1 Human)
UseTagsGFP and HAExpressionMammalianMutationE96GPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EEVEE-CaaX
Plasmid#155225PurposeMPAct with increased linker flexiblityDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-bjGFP
Plasmid#67032PurposePlasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsEYFP and SNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-set2
Plasmid#82546PurposePmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAP-AAV.FUS.3
Plasmid#222333PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.DepositorInsertAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
UseAAVTagsExpressionMutationPromoterp41Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC236
Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC235
Plasmid#216372PurposexhNup155-Nb3i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb3i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC227
Plasmid#216351PurposexhNup35-Nb1t (+3Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plYJP022-int7
Plasmid#202287PurposePlasmid containing integrase for LPattB7 siteDepositorInsertint7
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCD4 e/p Sal-(hCD2)
Plasmid#24070DepositorInsertCD2 (CD2 Human)
UseTagsmCD 4 promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB-1753-Nluc
Plasmid#158603PurposePlasmid expressing KB-1753-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #1
Plasmid#30319DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC1821_pCR8-SMN2-DN-RG6-SA
Plasmid#118655PurposeTransient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway DonorDepositorInsertSMN2-DN-RG6-SA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF CREB M1
Plasmid#22969DepositorInsertCREB (Creb1 Rat)
UseTagsFLAGExpressionMammalianMutationChanged Serine 133 to AlaninePromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy Flag GCN5
Plasmid#14106DepositorInsertGCN5 (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationE189G and K195NPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2xVlk1-3xFlag
Plasmid#172773PurposemRNA synthesis for injection into embryosDepositorInsertVlk1 (pkdcc.1.S Frog)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #2
Plasmid#30320DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαq*-BERKY3
Plasmid#158425PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
negMaLionB
Plasmid#113911Purposenegative control for Blue ATP sensorDepositorInsertnegMaLionB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUSE-RapR-Src(KD)-myc
Plasmid#25934DepositorInsertRapR-Src (Fkbp1a Mouse)
UseTagsExpressionMammalianMutationThis pUSE-RapR-Src(KD)-myc plasmid contains the k…PromoterAvailable sinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z22
Plasmid#120065PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
hRIP1 HA GFP K45A
Plasmid#41389DepositorInsertRIP1 (RIPK1 Human)
UseTagsGFP and HAExpressionMammalianMutationKinase Domain Mutant (K45A)PromoterCMVAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
Plasmid#172535PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistanceDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCX-ThPDE5VV
Plasmid#134778PurposecGMP FRET biosensorDepositorInsertThPDE5VV
UseTagscpVenus-Venus and mTurquoise2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92390Purposeexpresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cellsDepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαi*-BERKY3
Plasmid#158424PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsmycExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOZC-PALB2
Plasmid#133552PurposePALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.DepositorInsertPALB2 (PALB2 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)
Plasmid#128608PurposeExpresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEF6 Vps53-V5-6His
Plasmid#175097PurposeV5 tagged Vps53 is expressed in mammalian cellsDepositorInsertVps53 (Vps53 Mouse)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoD-NAD
Plasmid#193826PurposeCMV overexpression of ChemoD-NAD in mammalian cell linesDepositorInsertChemoD-NAD
UseTagsExpressionMammalianMutationShadowG: T225R, HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)
Plasmid#179064PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuoleDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VAM3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL856
Plasmid#214256PurposeExpression TadDE-NG in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB-cHyPer2
Plasmid#84728PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxideDepositorInsertHyPer2
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only