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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Publication

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo VPS50-13Myc

Plasmid

#175093

Purpose

13Myc tagged VPS50 is expressed in mammalian cells

Depositor

Juan Bonifacino

Publication

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VPS50 (CCDC132 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-His6

Plasmid

#51536

Purpose

Express His6-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-GST

Plasmid

#51528

Purpose

Express GST-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-Vif

Plasmid

#46313

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
Vif DNA-1
linker
Vif DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
Setd3-Flag-pGEX4t-1

Plasmid

#49145

Purpose

bacterial expression of GST-Setd3-Flag

Depositor

Jing H Wang

Publication

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Tags

FLAG and GST

Expression

Bacterial

Promoter

tac

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7

Plasmid

#46306

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
DNA-1 site
linker
DNA-2 site

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Promoter

Tac

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSMP-MeCP2_3

Plasmid

#36379

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MeCP2 (MECP2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_1

Plasmid

#36335

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-SMYD2_1

Plasmid

#36349

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Publication

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-lyn C466A-GFP

Plasmid

#35038

Depositor

Anna Huttenlocher

Publication

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn C466A-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Publication

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

Plasmid

#104116

Purpose

synOptopatch construct

Depositor

Adam Cohen

Publication

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-H133Y-FLAG

Plasmid

#102324

Purpose

Human SIRT6 H133Y with FLAG tag for mammalian expression

Depositor

Hening Lin

Publication

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed histidine 133 to tyrosine

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

Plasmid

#69049

Purpose

lentiviral expression of IKZF2 variant 2 with FLAG tag

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 2

Promoter

CAG

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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