We narrowed to 29,591 results for: Ide

1. pLVX-mCherry-SPASTIN, F124D

   Plasmid

   #180648

   Purpose

   Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, F124D mutation, has siRNA resistan…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPS0385

   Plasmid

   #133235

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPS0386

   Plasmid

   #133236

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPS0383

   Plasmid

   #133233

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPS0380

   Plasmid

   #133230

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA-hNgAgo

   Plasmid

   #104384

   Purpose

   human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

   Insert

   hNgAgo
   

   Use

   Tags

   3XFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sema4g-AP-His

   Plasmid

   #72034

   Purpose

   Expresses the extracellular region of the Sema4G protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4g (Sema4g Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sema4f.a-AP-His

   Plasmid

   #72032

   Purpose

   Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4f.a (Sema4f Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Unc5a.z-AP-His

   Plasmid

   #72051

   Purpose

   Expresses the extracellular region of the Unc5A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5a.z (Unc5a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ntn3-AP-His

    Plasmid

    #71979

    Purpose

    Expresses the entire Netrin 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn3 (Ntn3 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Plxnb3-AP-His

    Plasmid

    #72004

    Purpose

    Expresses the extracellular region of the PlexinB3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb3 (Plxnb3 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-mCherry-SPASTIN, L177D

    Plasmid

    #180649

    Purpose

    Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, L177D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid

    #180653

    Purpose

    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GCACCCAUACCUUUACAUU

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 RPS6KL1 MIT, residues 20-140

    Plasmid

    #180623

    Purpose

    Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 20-140

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCA528 SPASTIN , F124D, residues 108-200

    Plasmid

    #180629

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, F124D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 SPASTIN , L177D, residues 108-200

    Plasmid

    #180634

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, L177D

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 USP8, residues 1-147

    Plasmid

    #180636

    Purpose

    Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP8 (USP8 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-147

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET16B VPS4A, residues 1-84

    Plasmid

    #180638

    Purpose

    Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Residues 1-84

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CALPAIN-7 residues 1-165

    Plasmid

    #180611

    Purpose

    Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 CALPAIN-7 residues 1-165, V18D

    Plasmid

    #180612

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, V18D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge