We narrowed to 2,146 results for: MP

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pTRIP-CMV-puro-2A-BLM

Plasmid

#127641

Purpose

Over-expression of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

BLM (BLM Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH

Plasmid

#105981

Purpose

This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

Depositor

Marcel Bruchez

Article

Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

Insert

hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH

Use

AAV

Tags

FAPdL5-POST, T2A-dTomato, and myc

Promoter

human synapsin 1

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-C-10

Plasmid

#56224

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP12-Fibrillarin-7

Plasmid

#56252

Purpose

Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Fibrillarin (FBL Human)

Tags

mIFP12

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo

Plasmid

#160092

Purpose

Lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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FH1-CLGT#2-mCherry-_PGK-H2B-CFP

Plasmid

#164106

Purpose

Fluorescent reporter for genetic tracing of classical Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

CLGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-Mito-7

Plasmid

#56231

Purpose

Localization: Mitochondria, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Mito (COX8A Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCLHCX-mPM20D1-flag

Plasmid

#84566

Purpose

mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

Depositor

Bruce Spiegelman

Article

Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

Insert

peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

Retroviral

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_eGFP (open)

Plasmid

#160094

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Pere Roca-Cusachs, Michael Sheetz

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
mIFP-Calnexin-N-14

Plasmid

#56214

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Calnexin (CANX Human)

Tags

mIFP

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP12-CD81-10

Plasmid

#56248

Purpose

Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD81 (CD81 Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pL40C_PGKintron_Cas9_Green

Plasmid

#134966

Purpose

Lentiviral vector coding Cas9 and mNeonGreen

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Insert

Cas9-P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

hPGK promoter with beta-globin intron

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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