We narrowed to 7,108 results for: Tet-on

1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

   Plasmid

   #129053

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA1 (mouseTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER-retro-puro-tetO-Mek1-shRNA

   Plasmid

   #53179

   Purpose

   Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (Map2k1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

   Plasmid

   #129029

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA1 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-His-TET1-878-911(pL91)

   Plasmid

   #124390

   Purpose

   E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911 wildtype (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500

   Plasmid

   #45778

   Depositor

   Richard Murray

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Tags

   YbaQ degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   PLlacO-1

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

   Plasmid

   #108482

   Purpose

   inducible expression of vsv-Mis12-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   Mis12-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by Mis12

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEST-PLtetO-1-UTR1-LacI-YbaQ-T500

   Plasmid

   #45776

   Depositor

   Richard Murray, Vincent Noireaux

   Insert

   Lac repressor
   

   Use

   Synthetic Biology

   Tags

   YbaQ degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   PLtetO-1

   Available since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

   Plasmid

   #52688

   Purpose

   Same as 41026, with additional linker

   Depositor

   Michael Nick Boddy

   Insert

   tetO7-Pcyc1-GST+LINKER
   

   Use

   Promoter replacement

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195622

   Purpose

   Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   Tight TRE promoter; Tet-ON (doxycycline)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXTK-BB4 [Backbone_repABC-pMlb-KanR-TetR_PartTypeBB]

    Plasmid

    #229390

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

    Plasmid

    #231601

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-TetO-Chim-Pchi-Q61A/E64M

    Plasmid

    #231521

    Purpose

    Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutations at position 61 Q to A and 64 E to…

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225645

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225648

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225647

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225646

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Use

    Tags

    HA signal FALG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-flag-Lag16-fibcon-iCAM1-tether

    Plasmid

    #205213

    Purpose

    This vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-TetOne-Puro-p62 K7A/D69A

    Plasmid

    #204549

    Purpose

    Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K7A/D69A

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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