We narrowed to 159,258 results for: addgene

1. sgRNA5_8xCTS-ECFP-pA

   Plasmid

   #200247

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 5

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA5_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sgRNA3_8xCTS-ECFP-pA

   Plasmid

   #200245

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 3

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA3_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA2_8xCTS-ECFP-pA

   Plasmid

   #200244

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 2

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA2_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgRNA1 _8xCTS-ECFP-pA

   Plasmid

   #200243

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 1

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA1_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. gRNA4 _8xCTS-ECFP-pA

   Plasmid

   #200246

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 4

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA4_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLIK BirA* zeo

   Plasmid

   #192332

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-BirA

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEN_TT BirA* w ccdB

   Plasmid

   #192310

   Purpose

   Gateway entry vector for cloning

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN_TT 3xFLAG-Linker

   Plasmid

   #192305

   Purpose

   Gateway entry vector for cloning

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   Linker
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus-Bik-L61G-pEGFP-C1

   Plasmid

   #177426

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Leucine 61 replaced with glycine

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid

    #177428

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycine 154 replaced with STOP codon

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NODAL-T7TS

    Plasmid

    #115250

    Purpose

    For in vitro transcription of human NODAL open reading frame RNA

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NODALvar-T7TS

    Plasmid

    #115251

    Purpose

    For in vitro transcription of a human NODAL splice variant open reading frame RNA

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL splice variant (NODAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H2B-MiniE-mEm

    Plasmid

    #181991

    Purpose

    MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    H2B-MiniE-mEm
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 6DUB (LOC149281)

    Plasmid

    #119967

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    LOC149281 (METTL11B Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 58-278

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. FOXN1 (FOXN1A-c017)

    Plasmid

    #110249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FOXN1 (FOXN1 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5T1G (CBX1)

    Plasmid

    #101884

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX1 (CBX1 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. 2QO2 (EPHA3)

    Plasmid

    #101271

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDHJS4 AN-

    Plasmid

    #78247

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pDHJS4 AN+

    Plasmid

    #78246

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only