We narrowed to 1,843 results for: rigi

1. p3xFLAG-CMV-Tlr4(Lps)

   Plasmid

   #27149

   Depositor

   Bruce Beutler

   Article

   Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

   Insert

   toll-like receptor 4(Lps) (Tlr4 Mouse)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed Proline 712 to Histidine. Native signal p…

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

2. RAMA-bio

   Plasmid

   #47786

   Purpose

   Expresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RAMA
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PTEX150-bio

   Plasmid

   #47788

   Purpose

   Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised PTEX150
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDual-eGFP(Stop66)

   Plasmid

   #63218

   Purpose

   Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Stop66)
   

   Use

   Lentiviral

   Tags

   T7 and his6

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MSP9-bio

   Plasmid

   #47740

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP9
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. P12-bio

   Plasmid

   #47725

   Purpose

   Expresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised P12
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CLAG3.2-bio

   Plasmid

   #47797

   Purpose

   Expresses enzymatically monobiotinylated full-length CLAG3.2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised CLAG3.2
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ASP-bio

   Plasmid

   #47745

   Purpose

   Expresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised ASP
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RAP1-bio

    Plasmid

    #47794

    Purpose

    Expresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAP1
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RhopH2-bio

    Plasmid

    #47798

    Purpose

    Expresses enzymatically monobiotinylated full-length RhopH2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RhopH2
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmEos2-Linker_PCNA-MutC

    Plasmid

    #98272

    Purpose

    for low level expression of mEos2-PCNA in mammalian cells

    Depositor

    Mike Heilemann

    Article

    Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PTRAMP-bio

    Plasmid

    #47793

    Purpose

    Expresses enzymatically monobiotinylated full-length PTRAMP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PTRAMP
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H101-bio

    Plasmid

    #47733

    Purpose

    Expresses enzymatically monobiotinylated full-length H101 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised H101
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RH1-bio

    Plasmid

    #47748

    Purpose

    Expresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RH1 (partial)
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNF-KB-Split PS Intein-C tTA

    Plasmid

    #242035

    Purpose

    C-terminal segment of a blue-light-controlled split PS Intein tTA gene expression system, co-expressing mCherry under an NF-KB-inducible promoter

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-C tTA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The CMV promoter in the original vector was repla…

    Promoter

    NF-KB

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE Flag HA ITPA

    Plasmid

    #237998

    Purpose

    Inducible lentiviral expression of ITPA

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ITPA (ITPA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE Flag HA MPG Catalytic Inactive

    Plasmid

    #237807

    Purpose

    Lentiviral vector expressing Catalytic Inactive MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDA2ScIfm

    Plasmid

    #193786

    Purpose

    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTATAA synthetic promoter carrying binding sites…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLA2ScIfmTcIfm

    Plasmid

    #193792

    Purpose

    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLLacO-1 and PLTetO-1

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge