We narrowed to 12,307 results for: BASE

1. pChimera

   Plasmid

   #61476

   Purpose

   Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   U6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0090

   Plasmid

   #181954

   Purpose

   Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   7xTOPFlash
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH136

   Plasmid

   #47554

   Purpose

   pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

   Insert

   URA3
   

   Use

   Pcr template

   Tags

   Expression

   Mutation

   Promoter

   Native URA3 promoter, -243 to -1.

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9-TPC

   Plasmid

   #61478

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CRY2-mCh-superPLDx30

   Plasmid

   #188988

   Purpose

   A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CRY2 and mCherry

   Expression

   Mammalian

   Mutation

   K109R, P245A, G328S, G381V, G429D

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN-PITCh-GFP

   Plasmid

   #127888

   Purpose

   Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   Puro-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUS_B4

   Plasmid

   #31021

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ALIX-His(1-868, P801G)

   Plasmid

   #186808

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   ALIX-His (1-868, P801G) (PDCD6IP Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-Cre

    Plasmid

    #50797

    Purpose

    Expression of Cre recombinase under the control of the UAS-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Cre recombinase
    

    Use

    Multi-species

    Tags

    Expression

    Mutation

    Promoter

    UAS-hsp70

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Blast-mNeon

    Plasmid

    #207676

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMK449 (BromoTag-mClover-BSD)

    Plasmid

    #214379

    Purpose

    BromoTag-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. UBQ10:sXVE:(MCS)-S11

    Plasmid

    #108179

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUS_B2

    Plasmid

    #31019

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eGFP L202 gRNA

    Plasmid

    #119132

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L202 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUS_B5

    Plasmid

    #31022

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUS_B6

    Plasmid

    #31023

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEGFP-biRhoBAST

    Plasmid

    #196348

    Purpose

    Expression of mEGFP mRNA with biRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-biRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-EF1a-PTDSS1-IRES-Puromycin

    Plasmid

    #134667

    Purpose

    Expresses EGFP-tagged Mitochondria-associated ER membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    PTDSS1 (PTDSS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. VV229: GCaMP6f in fck

    Plasmid

    #58514

    Purpose

    Expresses GCaMP6f under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    C-terminal FCYENEV (ER export motif); this was no…

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. qTAG-N-Blast-miRFP670nano3

    Plasmid

    #207680

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pTomatoSlicer

    Plasmid

    #221085

    Purpose

    Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFUS_B3

    Plasmid

    #31020

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFUS_B1

    Plasmid

    #31018

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGG-TurboID

    Plasmid

    #209391

    Purpose

    Transiently expressing TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK485 (3mAID-Hygro)

    Plasmid

    #214397

    Purpose

    3mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    3mAID-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEGFP-2xbiRhoBAST

    Plasmid

    #196349

    Purpose

    Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-2xbiRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBJ1

    Plasmid

    #167130

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCarmine in pRSETB

    Plasmid

    #109484

    Purpose

    bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

    Depositor

    Oliver Griesbeck

    Article

    Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

    Insert

    mCarmine
    

    Use

    Tags

    N-terminal histidine tag in vector

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPpT4- PGK1prom-TIR1-FLAG

    Plasmid

    #189724

    Purpose

    Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMK455 (dTAG-mClover-BSD)

    Plasmid

    #214385

    Purpose

    dTAG-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMK456 (dTAG-mCherry-BSD)

    Plasmid

    #214386

    Purpose

    dTAG-mCherry for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LLhG-pHG165a E62K

    Plasmid

    #90051

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

    Insert

    LLhG/E62K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E62K

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pdCas9+sgRNA expression vector precursor

    Plasmid

    #171798

    Purpose

    PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Scarlet I-FRET 10-NeonGreen

    Plasmid

    #139364

    Purpose

    FRET Standard

    Depositor

    Richard Day

    Article

    Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

    Insert

    Scarlet 10AA Neon green
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge