We narrowed to 6,480 results for: poly

1. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKM5

   Plasmid

   #154166

   Purpose

   Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKM28

   Plasmid

   #154165

   Purpose

   Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel1A
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-XYLT2-sgRNA

   Plasmid

   #154862

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting XYLT2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   XYLT2 sgRNA (XYLT2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDF11-SFPQ(214-535)

   Plasmid

   #135439

   Purpose

   Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET15b-GreBdm-D41N

   Plasmid

   #129691

   Purpose

   express E. coli GreB E82C/C68S/D41N

   Depositor

   Jeff Gelles

   Article

   Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GreB E82C C68S D41N (greB E. coli)
   

   Use

   Tags

   his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMH-HT_CtCHAD-H253A-R256A-R260A

   Plasmid

   #127788

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

   Plasmid

   #127789

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

    Plasmid

    #127792

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg3…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-N1 Dynamin1 P294A

    Plasmid

    #112102

    Purpose

    Mammalian expression of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P294A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psicheck-2-Mc2r

    Plasmid

    #48174

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of MC2R (MC2R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Aviv Regev, Joan Brugge

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psicheck2 PTEN 3'UTR

    Plasmid

    #50936

    Purpose

    reporter for PTEN RNAi

    Depositor

    Pier Pandolfi

    Article

    Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

    Insert

    PTEN 3'UTR (PTEN Human)
    

    Use

    RNAi

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-p58

    Plasmid

    #166942

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    GFP and chicken lysozyme signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 BATF3

    Plasmid

    #67050

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BATF3 (BATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pGEMHE-H2B-mClover3

    Plasmid

    #171489

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H2B (H2BC11 Human)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCellFree_G03 TFAM

    Plasmid

    #67064

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAM (TFAM Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    S115C

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCellFree_G03 EBNA1BP2

    Plasmid

    #67072

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pDECKO-mCherry TFRC_B

    Plasmid

    #78544

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pCellFree_G03 SMAD2

    Plasmid

    #67095

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 TYK2

    Plasmid

    #67133

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TYK2 (TYK2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    A435S

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pDECKO-mCherry MALAT1_Exon.2

    Plasmid

    #78541

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDECKO-mCherry MALAT1_Exon.4

    Plasmid

    #78543

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCellFree_G03 BMPR2

    Plasmid

    #67127

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pGEMHE-bNup98-DN-NLS-mClover3

    Plasmid

    #171495

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Nup98-DN (NUP98 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PCRII apoA-Ib

    Plasmid

    #121896

    Purpose

    Plasmid for making zebrafish apoA-Ib in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA1b (apoa1b Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PCRII apoBb.2

    Plasmid

    #121899

    Purpose

    Plasmid for making zebrafish apoBb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBb.2 (apobb.2 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PCRII apoA-IVb.3

    Plasmid

    #121905

    Purpose

    Plasmid for making zebrafish apoA-IVb.3 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    apoa4b.3 (apoa4b.3 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PCRII apoA-IVa

    Plasmid

    #121902

    Purpose

    Plasmid for making zebrafish apoA-IVa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA4a (apoa4a Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PCRII apoA-Ia

    Plasmid

    #121895

    Purpose

    Plasmid for making zebrafish apoA-Ia in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    apoA-Ia (apoa1a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDECKO-mCherry MALAT1_Enhancer.4

    Plasmid

    #78539

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge