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FP5369 KIF17MD-flag- FIREmate

Plasmid

#216724

Purpose

Expresses motor domain of KIF17 fused to FIREmate in mamallian cells

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

KIF17 (KIF17 Human)

Tags

FIREmate and Flag

Expression

Mammalian

Mutation

1-547

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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mVenusN-Cavin1

Plasmid

#108887

Purpose

Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

caveolae associated protein 1 (CAVIN1 Human, Synthetic)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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mTq2-Calmodulin

Plasmid

#198200

Purpose

FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

Calmodulin 2 (Calm2 Rat)

Tags

mTurquoise2

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin1

Plasmid

#108888

Purpose

Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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SIV_Gag_Pol

Plasmid

#236243

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Gag and Pol

Use

Lentiviral

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPing

Plasmid

#47100

Purpose

Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

Ping cDNA
mPing
hygromycin resistance gene

Use

Plant expression

Tags

GFP

Expression

Yeast

Promoter

CaMV35s and StUbi3

Available Since

Sept. 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3 Zscan4 promoter

Plasmid

#69249

Purpose

Luciferase reporter for Dux4 activation of Zscan4 transcription

Depositor

Stephen Tapscott

Article

Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

Insert

Zscan4 (ZSCAN4 Human)

Use

Luciferase

Mutation

The two single nucleotide mismatches found betwee…

Promoter

Zscan4

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

Plasmid

#204621

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFP5380 LAMP1-SBP- mCherry-FIREtag

Plasmid

#216725

Purpose

Expresses LAMP1-mCherry-FIREtag in mamallian cells

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

LAMP-1 (LAMP1 Human)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-HA-GOLPH3

Plasmid

#21687

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216

Plasmid

#167354

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MDFI

Plasmid

#207836

Purpose

Tagged MDFI for western blotting and imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

MDFI (MDFI Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

Plasmid

#155306

Purpose

Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA BFP

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ASF1B

Plasmid

#31205

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ASF1B (ASF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
hE-cadherin/α-catenin fusion-pcDNA3

Plasmid

#45771

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Human, Frog)

Expression

Mammalian

Mutation

Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1932

Plasmid

#79781

Purpose

High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple259

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple259 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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