We narrowed to 8,448 results for: Ott

1. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

   Plasmid

   #204621

   Purpose

   Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6/V5-DEST-ASF1B

   Plasmid

   #31205

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   ASF1B (ASF1B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. p5E-3x125bp-mnx1

   Plasmid

   #169043

   Purpose

   5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   Mnx1 enhancer (3x) (mnx1 Zebrafish)
   

   Use

   Gateway entry cloning vector

   Tags

   Expression

   Mutation

   Promoter

   3xMnx1

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MDFI

   Plasmid

   #207836

   Purpose

   Tagged MDFI for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFI (MDFI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-hRNF216

   Plasmid

   #167354

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGY577

   Plasmid

   #166663

   Purpose

   Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cre: E340A, D341A

   Promoter

   CMV

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFP5380 LAMP1-SBP- mCherry-FIREtag

   Plasmid

   #216725

   Purpose

   Expresses LAMP1-mCherry-FIREtag in mamallian cells

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   LAMP-1 (LAMP1 Human)
   

   Use

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

   Plasmid

   #71282

   Purpose

   Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Zebrafish expression with tol2

   Tags

   Expression

   Mutation

   Promoter

   HSP70l

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPing

   Plasmid

   #47100

   Purpose

   Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

   Depositor

   Wayne Parrott

   Article

   Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

   Inserts

   Ping cDNA
   mPing
   hygromycin resistance gene
   

   Use

   Plant expression

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   CaMV35s and StUbi3

   Available since

   Sept. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

    Plasmid

    #155306

    Purpose

    Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mKate2-P2A-APEX2-2xFYVE_hrs

    Plasmid

    #67663

    Purpose

    Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-2xFYVE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2PH-PLCd-APEX2-P2A-mKate2

    Plasmid

    #71284

    Purpose

    Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2PH-PLCd-APEX2-P2A-mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 Zscan4 promoter

    Plasmid

    #69249

    Purpose

    Luciferase reporter for Dux4 activation of Zscan4 transcription

    Depositor

    Stephen Tapscott

    Article

    Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

    Insert

    Zscan4 (ZSCAN4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    The two single nucleotide mismatches found betwee…

    Promoter

    Zscan4

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. hE-cadherin/α-catenin fusion-pcDNA3

    Plasmid

    #45771

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

    Promoter

    CMV

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

15. mVenusN-Cavin1

    Plasmid

    #108887

    Purpose

    Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    caveolae associated protein 1 (CAVIN1 Human, Synthetic)
    

    Use

    Tags

    mVenus1-155

    Expression

    Mammalian

    Mutation

    altered codon usage

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC-HA active Nedd8-P2A-Hygro

    Plasmid

    #124307

    Purpose

    Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Five C-terminal amino acids missing compared to w…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mVenusC-Cavin1

    Plasmid

    #108888

    Purpose

    Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
    

    Use

    Tags

    mVenus156-239

    Expression

    Mammalian

    Mutation

    altered codon usage

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ5080_pHR_PGK_sfGFP_CoV-F2

    Plasmid

    #155304

    Purpose

    SARS-CoV-2 fluorescent reporter 2

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2 (N, ORF1ab Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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