We narrowed to 1,962 results for: ogn

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sgPPP1R2

Plasmid

#227391

Purpose

Adhesion module, guide RNA recognizes PPP1R2

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

guide RNA targeting PPP1R2

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110.i1

Plasmid

#135481

Purpose

E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110.i1

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSETT4gap

Plasmid

#153413

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Expression

Bacterial

Promoter

gapDH promoter from Eggerthella lenta

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 RNAiR2

Plasmid

#51585

Purpose

expresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSETT4tip

Plasmid

#153414

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Expression

Bacterial

Promoter

tipA promoter from Streptomyces lividans

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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HCB1

Plasmid

#98582

Purpose

Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

HCB1

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S129N

Plasmid

#51587

Purpose

expresses RNAiR myc-tagged Rem2 non-GTP binding

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

S129N; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 L317G

Plasmid

#51586

Purpose

expresses RNAiR myc-tagged Rem2 non-CaM interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

L317G; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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