159,258 results

1. pLKO.DEST.hygro

   Plasmid

   #32685

   Purpose

   Lentiviral cDNA/shRNA gateway destination vectors

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFP-NS1

   Plasmid

   #85739

   Purpose

   expresses CFP-FLAG-tagged NS1 monobody.

   Depositor

   John O'Bryan

   Article

   Spencer-Smith et al Nat Chem Biol. 2016 Nov 7. doi: 10.1038/

   Insert

   FLAG-tagged NS1 monobody
   

   Use

   Tags

   ECFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGP67A-PSMA

   Plasmid

   #11524

   Depositor

   Pamela Bjorkman

   Article

   Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

   Insert

   prostate-specific membrane antigen (FOLH1 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   There is a short N-terminal truncation of this pr…

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-mStayGold-Blast

   Plasmid

   #227254

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1066

   Plasmid

   #143919

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC2 (NFATC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR_LG lenti-(empty)-del-CTS

   Plasmid

   #112895

   Purpose

   Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

   Depositor

   Paul Blainey

   Article

   Feldman et al bioRxiv 262121

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-ON-shCtrl (neo)

   Plasmid

   #192076

   Purpose

   Lentivirus for Dox-inducible expression of a non-targeting control shRNA

   Depositor

   Roland Friedel

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1 / Tet-responsive element

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. OPRM1-DuET

    Plasmid

    #213361

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRM1 (OPRM1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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