164,577 results

1. pEGFP‐RNASEH2B

   Plasmid

   #108697

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2B (RNASEH2B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

   Plasmid

   #102245

   Purpose

   Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

   Depositor

   Jacob Corn

   Insert

   dCas9-VPR-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-VPR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN1-CMV-H-sDarken

   Plasmid

   #184800

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-FKBP(M)x4

   Plasmid

   #85003

   Purpose

   Expresses EGFP-FKBP(M)x4 in yeast

   Depositor

   Benjamin Glick

   Article

   Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

   Insert

   EGFP-FKBP(M)x4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHyg-AID*-6FLAG

   Plasmid

   #99519

   Purpose

   C-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   6xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK349 (BSD-P2A-mAID-mCherry)

   Plasmid

   #121183

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172318

   Purpose

   CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. iBE4max YE1 NG CLYBL

   Plasmid

   #174570

   Purpose

   doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet ON

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD_sfGFP3TCG4TAG

    Plasmid

    #174518

    Purpose

    Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    sfGFP
    

    Use

    Tags

    6 x His

    Expression

    Bacterial

    Mutation

    Recoded. TCG and TAG codons were introduced in po…

    Promoter

    araBAD

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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