We narrowed to 1,414 results for: PUC57

1. pTS2163

   Plasmid

   #169632

   Purpose

   Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO7-PhCMVmin / PPGK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIK198

   Plasmid

   #65629

   Purpose

   "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE7

   Plasmid

   #79462

   Purpose

   sgRNA shuttle vector; module 2

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE9

   Plasmid

   #79465

   Purpose

   sgRNA shuttle vector; module 3

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGE11

   Plasmid

   #79467

   Purpose

   sgRNA shuttle vector; module 4E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. S8_attL1-N-term-EGFP-attL2

   Plasmid

   #128472

   Purpose

   EGFP entry clones

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   EGFP entry clones
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS1044

   Plasmid

   #63797

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

   Insert

   3xFLAG followed by KlLEU2
   

   Use

   E. coli cloning vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   none

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. LI B-C N-NES KOZ b c7

   Plasmid

   #54445

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   Nuclear Export Signal (NES) of heat stable inhibitor (PKI)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Xlone Puro SNAP-eGFP-BASU

   Plasmid

   #218452

   Purpose

   expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   SNAP-eGFP-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSK-mU6-Ccu-sgRNA-SV40-puro

    Plasmid

    #192130

    Purpose

    Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Ccu-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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