We narrowed to 13,947 results for: cas9

1. hRad51(A89E)–Cas9(D10A)

   Plasmid

   #125563

   Purpose

   Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(A89E)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-His:dCas9:nls:10P3

   Plasmid

   #135982

   Purpose

   Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   dCas9 fused to 10 copies of the P3 coiled-coil
   

   Use

   CRISPR

   Tags

   His

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

   Plasmid

   #196197

   Purpose

   Cas9 + gRNA expression to edit G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pED9x (dCas9-KRAB-mCherry)

   Plasmid

   #163956

   Purpose

   Lentiviral expression plasmid of sgRNA with mCherry

   Depositor

   Junwei Shi

   Article

   Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSaCas9-1xms2-2x3’UTR

   Plasmid

   #122946

   Purpose

   For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   SaCas9-MS2-HBB 3' UTR
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-SpCas9-HF1

   Plasmid

   #201952

   Purpose

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_CMV-Cas9-P2A-HygR

   Plasmid

   #164133

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

   Plasmid

   #192651

   Purpose

   3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-10xGCN4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSaCas9-1xPP7-2x3'UTR

    Plasmid

    #122947

    Purpose

    For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    SaCas9-PP7-HBB 3' UTR
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE EF1α dCas9-NED

    Plasmid

    #109369

    Purpose

    Lentiviral vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. dSpyCas9-p300-P2A-CASANOVA

    Plasmid

    #113129

    Purpose

    Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    dCas9-p300-P2A-CASANOVA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF1-alpha

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1- dCas9-2xNLS-EGFP

    Plasmid

    #74710

    Purpose

    Expresses dCas9-2xNLS-EGFP in mammalian cells

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    dCas9-2xNLS-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-sgAMPKa2-Cas9-GFP

    Plasmid

    #208050

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA2 (PRKAA2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-sgAMPKa1-Cas9-GFP

    Plasmid

    #208049

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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