We narrowed to 5,538 results for: pcas

1. pCAG-attBr-Cre-rc-attP

   Plasmid

   #51264

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-Cre-rc-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT344_pattB-pCA-GFP-pA-FRT5

   Plasmid

   #52550

   Purpose

   for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMACE DEST CMV SpCas9

   Plasmid

   #206268

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaSpeR4 mito roGFP2-Orp1

   Plasmid

   #65001

   Purpose

   Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PCAF (delta5-53a.a.)

   Plasmid

   #65388

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   PCAF (KAT2B Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   lost 13-159bp of the ORF

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCAGGS-FLAG-BMAL1-S513A

   Plasmid

   #186831

   Purpose

   Expresses FLAG-BMAL1-S513A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 513 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-FLAG-BMAL1-S515A

   Plasmid

   #186832

   Purpose

   Expresses FLAG-BMAL1-S515A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 515 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-FLAG-BMAL1-S516A

   Plasmid

   #186833

   Purpose

   Expresses FLAG-BMAL1-S516A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 516 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-FLAG-BMAL1-S519A

    Plasmid

    #186834

    Purpose

    Expresses FLAG-BMAL1-S519A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 519 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-Flag-S1pr1-R120A

    Plasmid

    #203707

    Purpose

    Overexpression of FLAG-S1PR1-R120A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-S1PR1-R120A (S1PR1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R120A

    Promoter

    Available since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-Flag-hsDicer (D1320A)

    Plasmid

    #41586

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1320A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528-AMOT(1-300)-sortase

    Plasmid

    #166706

    Purpose

    expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT aa 1-300
    

    Use

    Tags

    His-SUMO and Sortase tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAMBIA-FLS2prom::FLS2-3xmyc

    Plasmid

    #86158

    Purpose

    FLS2 expression

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    3xmyc

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA-T-intron(Neo)-G(LNL)

    Plasmid

    #36908

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-pac-T2A-iCre

    Plasmid

    #113849

    Purpose

    Puromycin resistance gene and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-GBP-dCas9-mRFP

    Plasmid

    #102933

    Purpose

    Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein) and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pX330-Flag-eSpCas9-D10A

    Plasmid

    #80449

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_D10A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid

    #107305

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2x NLS and FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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