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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,673 results for: sequence

Showing: 1681 - 1700 of 12673 results

  4. pET28-NFHΔ36-72

    Plasmid
    #232060
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 36-72
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232060-plasmid-map-sequence-id-464069

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  5. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

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  6. pET28-NFMtail

    Plasmid
    #232056
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232056-plasmid-map-sequence-id-464064

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  7. pMZ103-rbs1-m8

    Plasmid
    #204470
    Purpose
    This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58
    Inserts
    quorum sensing regulator LasR (lasR )
    eYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    LasR L128F-S129W mutation
    Promoter
    In an operon and PLas
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    204470-plasmid-map-sequence-id-403702

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  8. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid
    #231595
    Purpose
    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter
    Depositor
    Marvin Tanenbaum
    Article
    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.
    Insert
    Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231595-plasmid-map-sequence-id-459554

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  9. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-2xXbp1(S255A)

    Plasmid
    #231596
    Purpose
    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-2xXbp1(S255A) pause site under H1 promoter
    Depositor
    Marvin Tanenbaum
    Article
    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.
    Insert
    Kozak sequence-Tornado-2xALFATag-10xSunTag-2xXbp1(S255A) pause sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231596-plasmid-map-sequence-id-459551

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  10. 3xMS-TR-100-PURO HRD

    Plasmid
    #207582
    Purpose
    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    Use
    Tags
    3xMS2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207582-plasmid-map-sequence-id-429467

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  11. pKM 1996

    Plasmid
    #217825
    Purpose
    Expresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.
    Depositor
    Aziz Sancar
    Article
    Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.
    Insert
    SA-phr1
    Use
    Tags
    Maltose-binding protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217825-plasmid-map-sequence-id-434879

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  12. pSCL758

    Plasmid
    #219504
    Purpose
    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219504-plasmid-map-sequence-id-435244

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  13. pMAT_41BBICD

    Plasmid
    #197097
    Purpose
    This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for the intracellular domain of 4-1BB.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197097-plasmid-map-sequence-id-386833

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  15. pGG-GTS-TurboID-GFP

    Plasmid
    #209405
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    GTS-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209405-plasmid-map-sequence-id-418457

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Showing: 1681 - 1700 of 12673 results