159,332 results

1. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85008

   Purpose

   For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

   Plasmid

   #132773

   Purpose

   Expression of human LMNA.

   Depositor

   Jan Lammerding

   Article

   Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKH118_keima-YIPF4

   Plasmid

   #214971

   Purpose

   Golgiphagy flux reporter keima-YIPF4

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   keima-YIPF4 (YIPF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ECFP-TevS-YPET

   Plasmid

   #100097

   Purpose

   FRET-based ECFP-YPET reporter that is cleaved by TEV

   Depositor

   Jim Wells

   Article

   Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

   Insert

   ECFP-TevS-YPET
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. h-Menkes

   Plasmid

   #196953

   Purpose

   Plasmid expressing an antigen targeting the Menke's disease copper ATPase

   Depositor

   James Trimmer

   Insert

   Copper ATPase 1 (ATP7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 FLII12Pglu-700uDelta6

   Plasmid

   #17866

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLII12Pglu-700uDelta6
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   700uDelta6, ECFP insert in mglB

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF3022

   Plasmid

   #144498

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RXRG (RXRG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MGAT-SEP-mRuby3

    Plasmid

    #200937

    Purpose

    cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    MGAT2 (MGAT2 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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