We narrowed to 4,495 results for: Ado

1. EF1a_KLF17_P2A_Hygro_Barcode

   Plasmid

   #120503

   Purpose

   Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF17 (KLF17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a_SOX2_P2A_Hygro_Barcode

   Plasmid

   #120480

   Purpose

   Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.hSynapsin1.OxLight-ctr

   Plasmid

   #169793

   Purpose

   Control non-binding fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight-ctr
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E54K; T111A

   Promoter

   human Synapsin-1

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-CAG-ChR2-YFP

   Plasmid

   #204345

   Purpose

   PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   ChR2-YFP
   

   Use

   Tags

   Yellow fluorescent protein

   Expression

   Mammalian

   Mutation

   C-terminus (aa315-737) is deleted - PMID: 1461559…

   Promoter

   CAG

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEZYeGFP-SARS-CoV-2E

   Plasmid

   #224580

   Purpose

   To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

   Depositor

   Isabel Mérida

   Article

   Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

   Insert

   SARS-CoV-2E (E SARS-CoV-2)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EF1a_ETV2_P2A_Hygro_Barcode

   Plasmid

   #120436

   Purpose

   Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_TFAP2C_P2A_Hygro_Barcode

    Plasmid

    #120487

    Purpose

    Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a_CDX2_P2A_Hygro_Barcode

    Plasmid

    #120432

    Purpose

    Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTwist-WIV1-CoV Δ18

    Plasmid

    #164439

    Purpose

    Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    WIV1-CoV Spike truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF1a_ERG_P2A_Hygro_Barcode

    Plasmid

    #120434

    Purpose

    Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid

    #140163

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-2X-P2A-Puro

    Plasmid

    #110840

    Purpose

    Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-2X
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A and 2 tandem NLS sequences in the XTEN linker

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet-1 Flag-LplA

    Plasmid

    #232118

    Purpose

    For bacterial expression of Flag-tagged E. coli LplA.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    lplA (lplA Escherichia coli (strain K12))
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a_HAND2_P2A_Hygro_Barcode

    Plasmid

    #120447

    Purpose

    Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a_KLF1_P2A_Hygro_Barcode

    Plasmid

    #120488

    Purpose

    Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF1 (KLF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge