9,096 results

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pBabe-GFP-IKBalpha-mut (super repressor)

Plasmid

#15264

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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myrAkt delta4-129

Plasmid

#10841

Depositor

Richard Roth

Publication

Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

Insert

Akt (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Mutation

Deletion of PH domain (aa4-129)

Promoter

SV40

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pAV-U6+27-Tornado-Broccoli

Plasmid

#124360

Purpose

Overexpression of circular RNA (Broccoli)

Depositor

Samie Jaffrey

Publication

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-Broccoli

Expression

Mammalian

Promoter

U6+27

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Publication

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Promoter

Ubiquitin

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Publication

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTagRFP657-N1

Plasmid

#31959

Depositor

Vladislav Verkhusha

Publication

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Insert

TagRFP657

Expression

Mammalian

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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mCherry-UtrCH

Plasmid

#26740

Depositor

William Bement

Publication

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

mCherry

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pAL149

Plasmid

#22275

Purpose

Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PhyB(1-908) (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

mCherry-Kras4BCT (prenylation sequence)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-BP1-2 pLPC-Puro

Plasmid

#19835

Depositor

Titia de Lange

Publication

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

Fragment of p53-Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

mCherry

Mutation

Fragment of human 53BP1 aa 1220 - 1711

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Publication

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Tags

YFP

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHR-CMV-nlsCRE

Plasmid

#12265

Depositor

Didier Trono

Publication

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Publication

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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lentiGuide-Hygro-eGFP

Plasmid

#99375

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-eGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-smURFP

Plasmid

#80349

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP.

Depositor

Erik Rodriguez, Roger Tsien

Publication

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Insert

smURFP

Use

Lentiviral

Promoter

CMV

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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