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pBAD-WzxE-TEV-His10
Plasmid#226781PurposeExpression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coliDepositorInsertwzxE
UseTagsTEV cleavable 10xHistidine tagExpressionBacterialMutationvaline insertion in position 2 due to the cloning…PromoteraraBADAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FKBP-V5-PRRX1
Plasmid#215024PurposeAAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1DepositorInsertpaired related homeobox 1 (PRRX1 Human)
UseAAVTagsFKBP-V5ExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_miRFP670nano-P2A-ERM-gCam6f
Plasmid#214927PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)DepositorInsertmiRFP670nano-P2A-ERM-gCam6f
UseLentiviralTagsExpressionMammalianMutationPromoterCMVpAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1loop
Plasmid#215032PurposeMammalian expression of human TWIST1 (with NEUROD1 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROG2loop
Plasmid#215033PurposeMammalian expression of human TWIST1 (with NEUROG2 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_HAND2loop
Plasmid#215034PurposeMammalian expression of human TWIST1 (with HAND2 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_TAL1loop
Plasmid#215035PurposeMammalian expression of human TWIST1 (with TAL1 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1ins
Plasmid#215036PurposeMammalian expression of human TWIST1 (with insertion from NEUROD1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop with insertion from NEUROD1 bHLH…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROG2ins
Plasmid#215037PurposeMammalian expression of human TWIST1 (with insertion from NEUROG2)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop with insertion from NEUROG2 bHLH…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_HAND2ins
Plasmid#215038PurposeMammalian expression of human TWIST1 (with insertion from HAND2)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop with insertion from HAND2 bHLH l…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_TAL1ins
Plasmid#215039PurposeMammalian expression of human TWIST1 (with insertion from TAL1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop with insertion from TAL1 bHLH lo…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1M
Plasmid#215045PurposeMammalian expression of human TWIST1 (with fragment of loop from NEUROD1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationMiddle portion of TWIST1 bHLH loop (aa139, P) rep…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FKBP-V5-ALX1
Plasmid#215023PurposeAAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1DepositorInsertALX homeobox 1 (ALX1 Human)
UseAAVTagsFKBP-V5ExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant
Plasmid#215026PurposeFirefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutatedDepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
UseLuciferaseTagsExpressionMutation4X E-box mutations within Coordinator motifsPromoterSV40Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC2-gephyrin S268E/270E
Plasmid#199609PurposeEncodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.DepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationS268E/S270EPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2
Plasmid#165083PurposegRNA 2 of pair B for Stx5L-specific knockoutDepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1
Plasmid#165085PurposegRNA 1 of pair C for Stx5L-specific knockoutDepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2
Plasmid#165086PurposegRNA 2 of pair C for Stx5L-specific knockoutDepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ 3m shRNA-1
Plasmid#160067Purpose3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGGcTGaTTaCAACAAGGATTG (mutations shown in lower c…Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA3
Plasmid#160213PurposeKnock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGTATGACCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA2
Plasmid#160211PurposeKnock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGACGTTGCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA1
Plasmid#160209PurposeKnock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CCDC-bio-His
Plasmid#53331PurposeExpresses full-length Coiled-coil domain-containing protein 109A precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCCDC (MCU Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
G6f-bio-His
Plasmid#53342PurposeExpresses full-length Lymphocyte antigen 6 complex locus protein G6f precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertG6f (LY6G6F Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCBL-bio-His
Plasmid#51867PurposeExpresses full-length Discoidin, CUB and LCCL domain-containing protein 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertDCBL (DCBLD2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ESAM-bio-His
Plasmid#51751PurposeExpresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertESAM (ESAM Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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3X FLAG-FUS-WT
Plasmid#44985DepositorInsertFUS (Full length) (FUS Human)
UseTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only
