We narrowed to 4,784 results for: Mos
-
Plasmid#229845PurposeA CAGGS driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling follwed by and IRES and a myristoylated TagBFP for labeling the cell membrane.DepositorInsertLamin nanobody
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nmEML-mRFP
Plasmid#231178PurposeStabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFPDepositorInsert20nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-30nmEML-mRFP
Plasmid#231179PurposeStabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFPDepositorInsert30nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-15nmEML-mRFP
Plasmid#231177PurposeStabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFPDepositorInsert15nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
macroH2A1.1-GFP (pc2188)
Plasmid#223707PurposeExpression of macroH2A1.1 tagged N-terminal to GFPDepositorInsertmacroH2A1.1-GFP (Macroh2a1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
macroH2A1.2-GFP (pc2189)
Plasmid#223708PurposeExpression of macroH2A1.2 tagged N-terminal to GFPDepositorInsertmacroH2A1.2-GFP (Macroh2a1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218911PurposePiggyBac transpositionDepositorInsertV5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
UseTagsExpressionMammalianMutation2363G>C and 4155C>T and 4729T>G and 4731…PromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-mRuby3
Plasmid#166142PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.DepositorTypeEmpty backboneUseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
Plasmid#208977PurposeEntry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)DepositorInsertpUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
UseCRISPRTagsBPNLSExpressionMammalianMutationPromoterhuman U6Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)
Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)
Plasmid#208971PurposeRecruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G
Plasmid#194462PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G
Plasmid#194460PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-QF2 HDR plasmid
Plasmid#141099PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2z-lmo2
Plasmid#164657Purposefor the synthesis of zebrafish lmo2 mRNADepositorInsertlmo2 (lmo2 Zebrafish)
UseMrna synthesisTagsExpressionMutationPromoterSP6Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pLENTI-CAG-IRES-GFP
Plasmid#177005PurposeMammalian lentiviral expression vector encoding ETS2DepositorInsertETS2 (ETS2 Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CYYR1_pcDNA6.2/EmGFP-Bsd
Plasmid#176986PurposeMammalian expression vector encoding CYYR1 and EmGFP-BsdDepositorInsertCYYR1 (CYYR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBR3_pcDNA6.2/EmGFP-Bsd
Plasmid#176961PurposeMammalian expression vector encoding CBR3 and EmGFP-BsdDepositorInsertCBR3 (CBR3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLDN8_pcDNA6.2/EmGFP-Bsd
Plasmid#176963PurposeMammalian expression vector encoding CLDN8 and EmGFP-BsdDepositorInsertCLDN8 (CLDN8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM207A_pcDNA6.2/EmGFP-Bsd
Plasmid#176950PurposeMammalian expression vector encoding FAM207A and EmGFP-BsdDepositorInsertFAM207A (C21orf70 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
Plasmid#91806PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationResidues A1529-G1534 replaced with a PreScission …PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
Plasmid#91807PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease siteDepositorInsertRPO21 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationPreScission Protease site (LEVLFQGP) inserted bet…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (G112P)
Plasmid#135496PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationG112PPromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (L156P)
Plasmid#135497PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationL156PPromoterCMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)
Plasmid#135501PurposeMammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)DepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationY84C/C121S/A139CPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (I52P)
Plasmid#135486PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationI52PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (Y59P)
Plasmid#135492PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationY59PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (D61P)
Plasmid#135493PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationD61PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (V67P)
Plasmid#135494PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationV67PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (Y84P)
Plasmid#135495PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationY84PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (E166P)
Plasmid#135498PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationE166PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154A/H250Y
Plasmid#108491Purposeexpression of EGFP-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsEGFPExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-INCENP d543-746
Plasmid#108473Purposeexpression of vsv-INCENP d543-746DepositorInsertINCENP d543-746 AA (INCENP Human)
UseTagsVSVExpressionMammalianMutationDelta Alpha helix, aa543-746 deletedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shGBP1.2.mKO2
Plasmid#85210PurposeTRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a I18A
Plasmid#40043DepositorInserthSlp4-a I18A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationIsoleucine 18 to AlaninePromoterCMV promoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a linker
Plasmid#40040DepositorInserthSlp4-a linker (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationlinker domain (144-353)PromotercmvAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
5` CC: Puro – loxP – GFP-C
Plasmid#219563Purpose5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.DepositorInserthPGK-promoter_PuroR_2A_loxP_GFP-C
UseUnspecifiedTagsExpressionMutationPromoterhPGKAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:Beta2meGFP
Plasmid#45096DepositorInsertnAChR beta2 (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationThere are strain differences between C57BJ/6 and …PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only