We narrowed to 23,118 results for: Sis
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Plasmid#168980PurposeAMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCAP03-ermE*p
Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyMutationbla (amp resistance gene)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBA334
Plasmid#214746PurposeHigh copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CALPAIN-7-mCherry
Plasmid#180652PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Ap1g1-FKBP
Plasmid#46945PurposeCo-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
TagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateMutationL22F F31SPromoterTEF1Available SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB3-B-TelR
Plasmid#204992PurposeLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
TagsRbcS targeting sequenceExpressionPlantPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b WT
Plasmid#118356PurposeThis plasmid expresses human HSF4b WT.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
TagsGFP-HisExpressionBacterialPromoterT7Available SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH3
Plasmid#168979PurposeAMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
ExpressionInsectAvailable SinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8
Plasmid#124861PurposeMutagenesis of Slc17a8DepositorInsertSlc17a8 (Slc17a8 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVpuro
Plasmid#134983PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB-sSypHer (RbcS-SypHer)
Plasmid#84732PurposeExpresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stomaDepositorInsertsSypHer
TagsRbcS targeting sequenceExpressionPlantPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
TagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialPromotertacAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDGE331
Plasmid#153240PurposeM1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
TagsSuper ecliptic pHlorin A227DExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtMIR390a-NbSu-2
Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
ExpressionPlantPromoter2x35SAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T38S38
Plasmid#49524PurposeAmpicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertconstitutive TetR38
PromoterPtb38Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P750
Plasmid#49525PurposeAmpicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertP750
PromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BD-cop1at(335-675)
Plasmid#44973DepositorInsertBD-COP1 (COP1 Mustard Weed)
TagsGAL4 DNA-binding domainExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMVAvailable SinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
Available SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero9
Plasmid#187927PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero9DepositorInsertHero9
TagsFlag-6xHisExpressionBacterialAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB1-B-TelR
Plasmid#204980PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLXIN-Ap1g1-FKBP
Plasmid#46946PurposeRetroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseRetroviralTagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianAvailable SinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
RS17060_pMQ30_KO_Kan
Plasmid#185396PurposeNon-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11DepositorInsertsRR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region
Available SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS17055_pMQ30_KO_Kan
Plasmid#185398PurposeNon-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11DepositorInsertsRR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region
Available SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only