We narrowed to 81,735 results for: TRI

Showing: 4301 - 4350 of 81735 results

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RNAPII_CTD38-52

Plasmid

#98685

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 38-52, residues 1853-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-eGFP

Plasmid

#136098

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_eGFP

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-mTurquoise2

Plasmid

#136100

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mTurquoise2

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS_GUS

Plasmid

#136084

Purpose

For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_GUS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_eGFP-linker

Plasmid

#136086

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_eGFP(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_mScarletI-linker

Plasmid

#136088

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mScarletI(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB423

Plasmid

#68688

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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LovTAP N538E

Plasmid

#61431

Purpose

Expresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.

Depositor

Tobin Sosnick

Article

Strickland et al Nat Methods. 2010 Aug;7(8):623-6. doi: 1

Insert

LovTAP N538E

Tags

CBP

Expression

Bacterial

Promoter

T7 lac operator

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN2

Plasmid

#96977

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN1

Plasmid

#96976

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Cav (61-101)

Plasmid

#22447

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (61-101)

Tags

GST

Expression

Bacterial

Available Since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only
GST-Cav (135-178)

Plasmid

#22448

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin 1

Tags

GST

Expression

Bacterial

Mutation

aa 135-178

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-S75F

Plasmid

#24580

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc Caronte (CT#612)

Plasmid

#13861

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

myc

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFLAG Caronte (CT#304)

Plasmid

#13860

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

flag

Expression

Mammalian

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-37

Plasmid

#98686

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 27-37, residues 1773-1852

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB223

Plasmid

#68614

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTJK517

Plasmid

#121474

Purpose

CEN TRP1 CNA1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1

Use

Yeast cen vector

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot3

Plasmid

#79250

Purpose

Expresses EGFP-tagged DmNot3 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot3 (Not3 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC84-SEIPIN1

Plasmid

#96979

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
myc GbetaL mutant 5

Plasmid

#8479

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

G192D

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB105-mTalin

Plasmid

#100584

Purpose

Binary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Citrine-mTalin (Tln1 Mouse)

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCaMP5

Plasmid

#78705

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker

Depositor

Sebastian Lourido

Article

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Insert

GCaMP5

Promoter

SAG1 5' UTR

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLRP11minor.MYC

Plasmid

#131195

Purpose

Mammalian expression of human NLRP11 188A

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188A (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188A

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pet28bZFCNGA5

Plasmid

#69858

Purpose

His tagged protein for antibody production screening

Depositor

Gary Matthews

Article

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
peT3a-AqAdk_MVGDH

Plasmid

#18092

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Tags

6xHis

Expression

Bacterial

Mutation

Tyr 52 changed to Cys, Val 145 changed to Cys, …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pCDH-SIRT6-G60A

Plasmid

#102326

Purpose

Human SIRT6 G60A for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

changed glycine 60 to alanine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 7

Plasmid

#8481

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

F320S

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
myc GbetaL mutant 2

Plasmid

#8478

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

S72D

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
unc5bCD4d3+4-blac

Plasmid

#36149

Purpose

EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-blac tag

Depositor

Gavin Wright

Article

Sollner et al Genome Biol. 2009;10(9):R99. Epub 2009 S

Insert

unc5b (unc5b Zebrafish)

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Mutation

L2V

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 1

Plasmid

#8477

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

D42A

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 6

Plasmid

#8480

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Tags

myc

Expression

Mammalian

Mutation

T208D

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pJB128 (MatP-mEos2)

Plasmid

#72653

Purpose

Inducible expression of MatP-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

MatP-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSTAP-seq_fly-ctrl

Plasmid

#86380

Purpose

Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PI(DM)-Rac1(WT)dc1.FLARE

Plasmid

#87589

Purpose

PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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