We narrowed to 10,971 results for: NSI;

1. p-mCherry-C1-MerMAID2

   Plasmid

   #126514

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-mCherry-C1-MerMAID5

   Plasmid

   #126517

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID5
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p-mCherry-C1-MerMAID3

   Plasmid

   #126515

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID3
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p-mCherry-C1-MerMAID6

   Plasmid

   #126518

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID6
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p-mCherry-C1-MerMAID4

   Plasmid

   #126516

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID4
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p-mCherry-C1-MerMAID7

   Plasmid

   #126519

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID7
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-mCherry-C1-MerMAID1

   Plasmid

   #126513

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID1
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. rat brain inositol 1,4,5-trisphosphate 3-kinase A

   Plasmid

   #122155

   Purpose

   rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

   Depositor

   Christophe Erneux

   Article

   Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

   Insert

   inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJC20cdc8.27

   Plasmid

   #99362

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glutamic acid 129 to Lysine

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCh-1522

    Plasmid

    #82428

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMCh-1526

    Plasmid

    #82429

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+MLS-HyPer7

    Plasmid

    #136470

    Purpose

    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Tandem mitochondrial targeting signal of cytochro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid

    #229795

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2+IMS-HyPer7

    Plasmid

    #136469

    Purpose

    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    SMAC/DIABLO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Farnselation tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-iGluSnFR4s

    Plasmid

    #234443

    Purpose

    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge