Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,703 results for: NUC

Clear keywords
Showing: 1721 - 1740 of 12703 results

  3. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109426-plasmid-map-sequence-id-212924

    Enlarge

  4. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

    Enlarge

  6. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109430-plasmid-map-sequence-id-212945

    Enlarge

  7. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    John Diffley
    Article
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169164-plasmid-map-sequence-id-333982

    Enlarge

  8. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

    Enlarge

  9. pSico-U6-tornado:6xMBS:8G8ApA-syn-GFP-TVA-oG-WPRE-bGHpA

    Plasmid
    #231013
    Purpose
    Lentiviral helper virus for G-deleted rabies tracing, with U6-driven transcript amenable for nuclear capture in the presence of RVdG expressing MS2-KASH.
    Depositor
    Tomasz Nowakowski
    Article
    Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.
    Inserts
    Enhanced GFP
    TVA (CD320 Chicken)
    optimized G
    Tornado expression system containing 6X MS2 binding sites and 8G8A synthetic poly A capture sequence
    Use
    Lentiviral
    Promoter
    human U6 and human synapsin I
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231013-plasmid-map-sequence-id-459298

    Enlarge

  10. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

    Enlarge

  11. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

    Enlarge

  13. Monomer NK

    Plasmid
    #31178
    Depositor
    Feng Zhang
    Insert
    Monomer-NK
    Expression
    Bacterial
    Available Since
    July 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. FusionRed-FTR940-N-11

    Plasmid
    #62737
    Purpose
    Localization: Nuclear - Nucleolus, N1 Cloning Vector, Excitation:580, Emission:608
    Depositor
    Michael Davidson
    Insert
    FTR940
    Tags
    FusionRed
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pCAG-B19N

    Plasmid
    #59924
    Purpose
    Expression vector for Rabies virus SAD B19 nucleoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus nucleoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59924-plasmid-map-sequence-id-94984

    Enlarge

  17. pHS3

    Plasmid
    #81038
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    NATMX - HO endonuclease
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. mApple-FTR-940

    Plasmid
    #54902
    Purpose
    Localization: Nuclear - Nucleolus, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    FTR
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. tdTomato-FTR-940

    Plasmid
    #58094
    Purpose
    Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    FTR
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1721 - 1740 of 12703 results