We narrowed to 13,947 results for: cas9

1. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-SpCas9D10A nickase

   Plasmid

   #216737

   Purpose

   Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMVd1

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-evoCjCas9-BlastR

   Plasmid

   #194059

   Purpose

   Mammalian expression of evoCjCas9 nuclease

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-Dual-iCas9-hALB

   Plasmid

   #167502

   Purpose

   Expression of dual inducible Cas9 with hALB promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   hALB

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pspCas9-HF-tetra-com-vector

    Plasmid

    #132555

    Purpose

    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster vasa

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid

    #192663

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid

    #100936

    Purpose

    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-EFS-dCas9-GFP

    Plasmid

    #64104

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pinducer20-dCas9-SunTag-2A-BFP

    Plasmid

    #115779

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    dCas9-SunTag-2A-BFP
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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