We narrowed to 13,711 results for: cas9

1. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

   Plasmid

   #222992

   Purpose

   Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Chicken ß-actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS34.EF1α<GFP-2A-Cas9-RC

   Plasmid

   #207404

   Purpose

   Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Cas9-RC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP-2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(RBSmut)_Psyn-sgRNA500

   Plasmid

   #149576

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUbi_dCas9-BFP-KRAB-gRNA

   Plasmid

   #221503

   Purpose

   CRISPRi machinery fish codon optimized expressing plasmid

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Zebrafish Ubiquitin promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202561

   Purpose

   hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHDE-35S-Cas9-mCherry

   Plasmid

   #78931

   Purpose

   For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

   Depositor

   Yunde Zhao

   Article

   Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222501

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLM-AMA18.0 dCas9-VPR

    Plasmid

    #138945

    Purpose

    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

    Insert

    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    

    Use

    CRISPR and Synthetic Biology; Ama1 autonomously r…

    Tags

    NLS

    Expression

    Mutation

    Promoter

    p40S (AN0465) Aspergillus nidulans

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH-Ubi-miniSdd7-nCas9-PBE

    Plasmid

    #204856

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1, OsU3, 35S

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-CAG-hCAS9-neo

    Plasmid

    #166026

    Purpose

    AAVS1 targeting vector carrying CAG-SpCas9-neo

    Depositor

    Kosuke Yusa

    Insert

    AAVS1-CAGCas9IRESneo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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