We narrowed to 13,170 results for: ckb

1. pGWB535

   Plasmid

   #74873

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV126

   Plasmid

   #63192

   Purpose

   pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LC201: pMVP/PB/Puro-DEST

   Plasmid

   #121874

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SlugCas9-HF

   Plasmid

   #163796

   Purpose

   Expresses highly specific SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SlugCas9 (R247A, N415A, T421A, R656A)

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBait

   Plasmid

   #165146

   Purpose

   Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMY-empty-MCS

   Plasmid

   #163351

   Purpose

   The empty pMY backbone with multiple cloning site

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB59 pDEST crybb1:mKate2

   Plasmid

   #195986

   Purpose

   Transgenesis backbone

   Depositor

   Markus Affolter, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV125

   Plasmid

   #63191

   Purpose

   pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. yTREX

   Plasmid

   #177306

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. RT3GEPIR

    Plasmid

    #111169

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. P[acman]-Alpha2

    Plasmid

    #165859

    Purpose

    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAgR_Neo

    Plasmid

    #102992

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCardinal-Lifeact-7

    Plasmid

    #54663

    Purpose

    Localization: Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mCardinal-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pVk7-33G1-Flag

    Plasmid

    #58950

    Purpose

    backbone for mouse recombinant antibody with added C-terminal FLAG tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAtC

    Plasmid

    #78097

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Basta resistance

    Depositor

    Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NLS-HA

    Expression

    Plant

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-tLuc-mCherry

    Plasmid

    #107553

    Purpose

    tLuc-mCherry in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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