We narrowed to 16,820 results for: URE

1. pEGFPc1 IP3R3 WT

   Plasmid

   #236453

   Purpose

   transient overexpression of IP3R3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

   Insert

   IP3R3 (Itpr3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3HAT1Luc1

   Plasmid

   #236275

   Purpose

   Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

   Insert

   angiotensin II type 1 receptor (AGTR1 Human)
   

   Use

   Tags

   3xHA and Rluc8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2K7-bsd-UBI-tagRFP-KDEL

   Plasmid

   #114179

   Purpose

   Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Lentiviral

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Puro-AAVR-FLAG

   Plasmid

   #166716

   Purpose

   Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   AAVR-FLAG (KIAA0319L Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-FLAG-Jhdm2a

   Plasmid

   #38136

   Depositor

   Toru Nakano, Toshinobu Nakamura

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a (Kdm3a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-FLAG-Jhdm2a-H1122A

   Plasmid

   #38140

   Depositor

   Toru Nakano, Toshinobu Nakamura

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a-H1122A (Kdm3a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115; H1122A

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

    Plasmid

    #207953

    Purpose

    nuclease inactive control for TRF1FokI WT

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI (D450A)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Plasmid

    #100798

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    TVA, EGFP, tet transactivator
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

    Plasmid

    #207832

    Purpose

    telomere specific nuclease to create double-strand breaks at telomeres

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid

    #233348

    Purpose

    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted (Cyb5a 95-134) and N538E

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW031

    Plasmid

    #231144

    Purpose

    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

    Depositor

    Philip Cole

    Article

    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

    Insert

    S. aureus Sortase A mutant
    

    Use

    Tags

    Nostoc punctiform split intein C-terminal fragmen…

    Expression

    Bacterial

    Mutation

    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

    Promoter

    T7

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. ORAI1-YFP L273D-L276D

    Plasmid

    #114182

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP L273D-L276D (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    L273D/L276D

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ORAI1-YFP V107M

    Plasmid

    #114180

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP V107M (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    V107M

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV phSyn1(S)-tdTomato-WPRE

    Plasmid

    #51506

    Purpose

    Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV5

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HR180-LGR5-iCT

    Plasmid

    #129094

    Purpose

    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid

    #209298

    Purpose

    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.

    Depositor

    Birgitt Schuele

    Article

    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1

    Inserts

    dCas9
    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-inducible promoter (TRE3G)

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGImC_V5-CSNK1A1

    Plasmid

    #92014

    Purpose

    Expresses V5-CSNK1A1, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

    Plasmid

    #168107

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    Cas9-P2A-mCherry
    

    Use

    CRISPR and Cre/Lox

    Tags

    HA

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJM1-FH-AGO2-WT

    Plasmid

    #91978

    Purpose

    Expresses FLAG-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge