We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. pISL1.1.0-gDNA

   Plasmid

   #113788

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ISL1

   Depositor

   Kevin White

   Insert

   ISL1 (ISL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEHF.1.0-gDNA

   Plasmid

   #113785

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor EHF

   Depositor

   Kevin White

   Insert

   EHF (EHF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFOXF2.1.0-gDNA

   Plasmid

   #113784

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXF2

   Depositor

   Kevin White

   Insert

   FOXF2 (FOXF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHOXB5.1.0-gDNA

   Plasmid

   #113783

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB5

   Depositor

   Kevin White

   Insert

   HOXB5 (HOXB5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHOXB13.1.0-gDNA

   Plasmid

   #113782

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB13

   Depositor

   Kevin White

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLIS3.1.0-gDNA

   Plasmid

   #113780

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

   Depositor

   Kevin White

   Insert

   GLIS3 (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSRF.1.0-gDNA

   Plasmid

   #112458

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SRF

   Depositor

   Kevin White

   Insert

   SRF (SRF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEAF1.2.1-gDNA

   Plasmid

   #112449

   Purpose

   CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1

   Depositor

   Kevin White

   Insert

   DEAF1 (DEAF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pE2F5.1.0-gDNA

   Plasmid

   #112447

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

   Depositor

   Kevin White

   Insert

   E2F5 (E2F5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDLX1.1.0-gDNA

    Plasmid

    #112439

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1

    Depositor

    Kevin White

    Insert

    DLX1 (DLX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBP1.1.0-gDNA

    Plasmid

    #112432

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1

    Depositor

    Kevin White

    Insert

    HBP1 (HBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pATF1.1.0-gDNA

    Plasmid

    #112424

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1

    Depositor

    Kevin White

    Insert

    ATF1 (ATF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASB

    Plasmid

    #190175

    Purpose

    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

    Depositor

    Jessica Tyler

    Article

    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

    Insert

    sgRNA cloning site
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Added 5'-agagacc-3' upstream and 5'…

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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