Addgene

Showing: 1741 - 1760 of 1837 results

Results per page:

Sort results by:

pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule2-pCNF

Plasmid

#85495

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Use

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule2-Mb haloTyrRS C6

Plasmid

#160378

Purpose

C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyrosine tRNA synthatase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

L270S, Y271L, N311G, C313T

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG225 HIPK1 3'UTR mut

Plasmid

#12044

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR mut (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG177 HIPK1 3'UTR mut

Plasmid

#12035

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR mut (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Use

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Tags

Expression

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Tags

Expression

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Use

Tags

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
COC053

Plasmid

#209131

Purpose

Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

Depositor

Christopher A. O'Callaghan

Article

O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

Insert

Enzyme BirA (birA E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcpBpaRS_v2

Plasmid

#196485

Purpose

To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcpAzpaRS_v2

Plasmid

#196483

Purpose

To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule-Mb haloTyrRS C6

Plasmid

#160377

Purpose

C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyrosine tRNA synthatase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

L270S, Y271L, N311G, C313T

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Nuc-meCpG

Plasmid

#186464

Purpose

Expresses mCherry tagged methyl-CpG recognition motif targeted to nucleus in mammalian cells.

Depositor

Chongli Yuan

Article

Zhao et al ACS Sens. 2021 Oct 22;6(10):3575-3586. d

Insert

dimeric MBD1 (MBD1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNAZeo(-)HSC73

Plasmid

#86030

Purpose

Expression of Heat shock chaperone 70 in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Heat-shock cognate protein 73 (HSPA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge