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Plasmid#164131PurposeExpresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)DepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111590PurposeAn AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G
Plasmid#204188PurposeDoxycycline inducible lentiviral vector for human NOTUM overexpressionDepositorInsertNOTUM (NOTUM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]
Plasmid#159899PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR
Plasmid#194925PurposemRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected hoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterhPGKAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP
Plasmid#201681PurposeLentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expressionDepositorInsertsSFTPC promoter region (2kb upstream)
TagRFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP
Plasmid#129111PurposeHomologous recombination vector for excision reporter 1 in the CLYBL locus.DepositorInsertLSL, eGFP, NLS-RFP
UseCre/Lox and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorInsertTTF1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G
Plasmid#204189PurposeDoxycycline inducible lentiviral vector for human GATA6 overexpressionDepositorInsertGATA6 (GATA6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR myc-MBP-SspB-iRFP-NLS
Plasmid#223286PurposeAn SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.DepositorInsertMyc-MBP-SspB-irFP-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-dTomato-2xrGBD
Plasmid#222638PurposeRho reporterDepositorInsertmiRFP680-NES-p2A-dTomato-2xrGBD
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre
Plasmid#112683PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinaseDepositorInsertiRFP713
UseAAVTags2A-iCre and Nuclear localization signalExpressionMutationPromoterCMV-IEAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-135 mRFP-3MREs (AAV-alpha)
Plasmid#206929PurposeAlpha cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterMP-135Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3
Plasmid#200635PurposeNegative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cellsDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT
Plasmid#60419PurposeMammalian Expression of ITSN DH/PH-tgRFPt-NanoDepositorInserthITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationhITSN 1159-1509 // SSPB Y11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q
Plasmid#60418PurposeMammalian Expression of Tiam DH/PH-tgRFPt-MicroDepositorInsertmTiam1-tgRFPt-SSPB R73Q
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationmTiam 64-437 // SSPB R73Q, Y11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)
Plasmid#65446PurposeExpression of ephrinB1 and RFP in mammalian cellsDepositorInsertephrin B1 (Efnb1 Mouse)
UseLentiviralTagsSV40-RFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G
Plasmid#204190PurposeDoxycycline inducible lentiviral vector for human TFAP2C overexpressionDepositorInsertTFAP2C (TFAP2C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only